肺癌组织芯片中Stat3、bcl-2和VEGF表达与临床病理的关系

目的：通过检测肺癌组织芯片中Stat3、bcl-2和VEGF的表达，探讨它们在肺癌的发生发展及浸润转移中的作用，为肺癌的早期诊断、预后判断提供一定的理论依据。方法：应用组织芯片技术及免疫组织化学方法检测正常肺组织，癌前病变、原发癌及淋巴结转移癌组织中Star3、bcl-2和VEC-F的表达情况，并分析与各临床病理参数之间的关系。结果：实验组中Stat3、bcl-2、VEGF的表达阳性率分别为65．17％、77．53％、79．77％，均显著高于正常对照组（P〈0．05）；Stat3表达阳性率与组织学分级、临床分期及淋巴结转移有关（均P〈0．05）；肺癌中Stat3与VEGF，bcl-2的表达呈明显正相关。结论：Stat3与肺癌的浸润和转移密切相关，因而Stat3可用于判断肿瘤预后的指标检测。

Biological Significance of Expression of Stat3, bcl-2 and VEGF in Lung Cancer Tissue Microarray

Objective:To explore the roles of Stat3,bcl-2 and VEGF in occurrence,development of lung cancer,by detecting the expression of them in a lung cancer tissue microarray and to supply a theoretical evidence for diagnosis and judgment of prognosis.Methods:We applied tissue microarray technique and SP immunohis tochemical method to detect Stat3,bcl-2 and VEGF protein expressions and analyzed their relationship with each other in a lung cancer tissue microarray containing normal lung tissue specimen,precancerous diseases,cancer specimen and metastasis specimen.Results:Stat3,bcl-2 and VEGF proteins were respectively detected in 65.17%,77.53% and 79.77% of all the 89 lung cancer tissues,which were higher than those of control group,and the differences were significant (P<0.05).The positive ratio of the expressions on Stat3 were significantly relate to histological differentiations,clinical stages and lymphoid metastasis of lung cancers(P<0.05).There was significantly positive association among stat3,bcl-2 and VEGF.Conclusion:Stat3 plays an important role in proliferation of tumor cells.Detected the marker will be important to estimate cancer progression.