siRNA沉默水通道蛋白-4表达及对脑出血后脑水肿损伤的影响

目的探讨siRNA沉默水通道蛋白-4（AQP-4）表达对颅脑损伤（TBI）后创伤性脑水肿的影响。方法90只雄性sD大鼠进行创伤性脑水肿模型制备，随机分为3组，TBI组大鼠不接受任何治疗；AQP-4siRNA组进行siRNA质粒干扰；空载质粒组处理方法同AQP-4siRNA组，但所转质粒不含AQP-4siRNA片段。而后观测大鼠神经功能缺损评分（NIHSS）、脑组织AQP-4mRNA的表达及脑组织含水量。结果治疗后第7、14、21、28天，3组NIHSS均有下降，AQP4siRNA组〈空载质粒组〈TBI组，差异均有统计学意义（均P〈0．05）。治疗后第1、3、5、7天，TBI组、空载质粒组AQP-4mRNA的持续高表达，而AQP-4siRNA组则明显改善（P〈0．05）。造模后3组大鼠脑组织含水量逐渐增加，第3天达到高峰，而后逐渐下降，TBI组与空载质粒组无明显差异（P〉0．05），而AQP-4siRNA组低于TBI组、空载质粒组（P〈0．05）。结论通过siRNA沉默AQP-4表达可明显改善TBI后创伤性脑水肿严重程度，缩短脑功能恢复时间。

Studies on siRNA interferes with aquaporin-4 expression and influences on traumatic brain injury

Objective To investigate the aquaporin-4 （AQP-4） effects and mechanism on traumatic brain edema. Methods 90 male SD rats were prepared traumatic brain edema, were randomly divided into three groups, TBI rats received no treatment; AQP-4 siRNA groups with siRNA plasmid interference; empty plasmid group with AQF-4 siRNA treatment group, but that does not contain AQP-4 siRNA transfeeted plasmid fragments. Then observed neurological function in rats （NIHSS）, brain tissue AQP-4 mRNA expression and brain water content. Results Mter 7,14,21,28 days treatment, three groups were decreased with NIHSS, AQP-4 siRNA group 〈 empty plasmid group 〈 TBI group, the differences were statistically significant （all P 〈0.05）. 1,3,5,7 days after treatment, TBI group, empty plasmid group AQP-4 mRNA continued high expression, whereas AQP-4 siRNA group was significantly improved （ P 〈 0.05 ）. After modeling three rats brain water con- tent increased gradually, the first three days reached a peak and then gradually decline, TBI group and empty plasmid group was no significant difference （ P 〉 0.05 ）, while AQP-4 siRNA group was lower than TBI group, empty plasmid group （ P 〈 0.05 ）. Conclusion By expressing siRNA silencing AQP-4 after TBI can significantly improve the severity of traumatic brain edema, brain function to shorten recovery time.