长期应用大黄对大鼠结肠肌电及Cajal间质细胞的影响

目的 研究长期服用刺激性泻剂对大鼠结肠肌电的影响，探讨Ｃａｊａｌ间质细胞（ＩＣＣ）在“泻剂结肠”肌电变化中的作用。方法 ３２只大鼠随机分为２组，实验组饲以含大黄饲料，３月后测定结肠慢波频率及振幅；用碘化锌－狐酸法（ＺＩＯ）观察肌间念念不忘爱射电镜观察肌间丛神经和ＩＣＣ的超微结构变化。结果 长期服用大黄后，大鼠结肠慢波频率明显减慢（Ｐ〈０．０５），肌间丛ＩＣＣ分布不均匀，突起连接杂乱；电锏上见肌间丛

Effects of long-term application of Rhubarb on electromyography and interstitial cells of Cajal of colon in rats

Objective To investigate the effects of long-term application of cathartics on electromyography of rat colon and to explore the role of interstitial cells of Cajal (ICC) in it. Methods Colonic slow waves of the rat was examined after the animals were fed for 3 months. ICC in myenteric plexus was observed by ZIO method. Meanwhile, the ultrastructural changes of the nerves and ICC were also observed. Results The frequency of slow waves of cathartic colon was reduced significantly (P<0.05). The distribution of ICC in myenteric plexus was asymmetric and the processes connected with each other. Vacuolar degeneration of axon and ICC-like cells was revealed by electron microscopy in myenteric plexus of cathartic colon. Conclusion Long-term application of Rhubarb can lead to reduced frequency of colonic slow waves. The possible mechanism is the degeneration of ICC and myenteric nerve plexus.