血管肽对人内乳动脉平滑肌细胞增殖及原癌基因表达的影响

目的 :观察血管肽 (AP)对血小板衍化生长因子 BB(PDGF- BB)刺激下人内乳动脉平滑肌细胞(h IMASMC)增殖及 c- m yb m RNA表达的影响 ,并探讨其作用机制。方法 :采用细胞计数及 3H- Td R掺入法观察细胞增殖及 DNA合成 ;以 N orthern blot观察 c- m yb m RN A表达。结果 :AP对 h IMASMC、DNA合成及 c- m ybm RNA表达具有剂量依赖性抑制作用。结论 :AP可抑制 h IMA SMC增殖 ,其作用与原癌基因表达有关

Effect of angiopeptin on proliferation and c-myb mRNA expression in human internal mammary artery smooth muscle cells

Objective:To analyze the effect o f angiopeptin (AP) on the proliferation and c-myb mRNA expression in culture d human internal mammary artery smooth muscle cells (hIMASMC) treated with plat elet-derived growth factor BB (PDGF-BB). Method:The hIMASMC were cultured. The cell proliferation a nd the DNA synthesis in hIMASMC were determined by cell counting and 3 H-TdR incorporation. The expression of c-myb mRNA of hIMASMC was detected by Nort hern blot. Result:AP produced a dose-dependent inhibition of pro liferation and DNA synthesis in hIMASMC. The c-myb mRNA expression of hIMASM C was also inhibited by AP in a dose-dependent manner. Conclusion:AP strongly inhibits proliferation, DNA syn thesis and c-myb mRNA expression in hIMASMC. It suggests that the mec hanism of AP on hIMASMC proliferation is partially related to suppressing the g rowth regulated gene expression.