大鼠脊髓前角内NMDA受体的定位与生后发育

目的：探讨NMDA受体亚基（NMAR1和NMDAR2A／B）在大鼠脊髓前角的细胞学定位和生后发育特征。方法：免疫组织化学染色体技术。结果：NMDAR1与NMDAR2A／B免疫反应产物丰富地分布在脊髓前角RexedⅨ和Ⅷ 层内，主要定位于神经元胞体、树突和轴突样纤维终末上，生后早期的NMDAR1和NMDAR2A／B表达具有明显的动态变化，生后7d（P7）的表达微弱，随后逐渐上调，至P21达高峰水平，然后维修此水平至成年。结论NMDAR1和NMDAR2A是构成脊髓前角神经元功能性NMDA受体的重要亚基，NMDA受体可能参与生后早期运动神经元成熟或可逆性调控过程。

LOCALIZATION OF NMDA RECEPTORS IN THE VENTRAL HORN =OF SPINAL CORD OF ADULT RAT AND DURING POSTNATAL DEVELOPMENT

Objective To investigate the cellular localization and developmental patterns of NMDA receptor subunits(NMDAR1 and NMDAR2A/B) in the ventra l horn of rat spinal cord during postnatal stages. Method Immunohistochemical staining method was used in the pres ent study. Results NMDAR1-or NMDAR2A/B-immunorectivity is abundantly observ ed in the lamina Ⅷ and Ⅸ of ventral horn.The immunopositive product is localiz ed mainly in the neuronal somata,dendrites and axon terminals.Dynamic changes of NMDA receptor expression occur in early postnatal stages.NMDA receptor subunits express weakly at postnatal 7 days (P7),goes up at P14,reaches maximum level at P21,then remains this level until adult. Conclusion Neuronal functional NMDA receptors are composed of NMDA R1 and NMDAR2A in heteromeric configuration in the ventral horn of spinal cord;N MDA receptors may be involved in motor neuron maturation and plasticity during p ostnatal development.