HBV携带产妇血清与乳汁HBV-DNA变量相关性探讨

目的分析HBV携带产妇血清与乳汁HBV-DNA变量相关性,为安全哺乳提供科学依据。方法 ELISA法检测HBV携带产妇血清HBV-M,荧光定量PCR检测血清与乳汁HBV-DNA,统计学方法分析两变量相关性。结果以乳汁HBV-DNA小于500拷贝/ml为阴性,其中A组(HBsAg、HBeAg、HBcAb阳性)乳汁阴性率为11.7%(15/128),B组(HBsAg、HBcAb阳性或HBsAg、HBeAg阳性)乳汁阴性率72.3%(11/15),C组(HBsAg、HBAbe、HBcAb阳性)乳汁阴性率94.9%(185/195),三组阴性率比较差异有统计学意义(P0.05)。248例HBV携带产妇同时检测血清和乳汁HBV-DNA,各组分组情况同上,A组血清HBV-DNA阳性率95.4%(83/87),乳汁HBV-DNA阳性率80.7%(71/87),两者阳性率比较,差异有统计学意义(P0.05);B组血清HBV-DNA阳性率为46.2%(6/13),乳汁HBV-DNA阳性率30.8%(4/13),差异无统计学意义(P0.05);C组血清与乳汁HBV-DNA阳性率分别为31.8%(37/148)和4.1%(6/148),差异有统计学意义(P0.05),每组两变量统计学分析正相关且相关程度高。结论不同感染模式的HBV携带产妇,血清与乳汁HBV-DNA病毒载量不同,HBV携带产妇哺乳前应该检测血清和母乳中的HBV-DNA,以指导母乳安全喂养。

Exploration of the correlation of the HBV-DNA loads in serum and breast milk in HBV carrier mothers

Objective To analyze the correlation of the HBV - DNA loads in serum and breast milk in HBV carrier mothers and provide a scientific basis for safety breast - feeding. Methods The hepatitis/3 virus marker （HBV- M） and HBV- DNA loads in the serum of HBV carrier mothers were detected by ELISA and FQ- PCR respectively. The correlation between the two variables was analyzed. Results The HBV- DNA in the breast milk lower than 500 copies/ml was thought to be negative. The negative rates of the HBV - DNA in the breast milk of Group A （ with positive HBsAg, HBeAg and anti - HBc）, Group B （with F~itive HBsAg and anti- HBc or positive HBsA~g and HBeAg） and Group C （with positive HBsAg, anti - HBe and anti - HBc） were 11.7 % （15/128）, 72.3 % （11/15） and 94.9 % （ 185/195 ）, respectively, which were statistically significant （ P 〈 0.05）. The HBV-DNA was simultaneously detected in the serum and breast milk of 248 IqBv carrier mothers. The positive rates of HBV- DNA in the ,serum and breast milk in Group A were 95.4% （83/87） and 80.7% （71/87）respectively, showing significant difference（ P 〈 0,05）. The rates in Group ]3 were 46.2 % （6/13） and 30.8 % （4/13）, without statistical difference（ P 〉 0.05）. The rates in Group C were 31.8 % （37/148） and 4.1% （6/148）, indicating significant difference （P 〈 0.05 ）. The two variables were positively and highly correlated for each group. Conclusions The HBV- DNA loads in the serum and breast milk are different among H13V carrier mothers with different infection modes. The loads should be determined in HBV carrier mothers in time in order to guide the safe breast - feeding.