乙型肝炎病毒核酸定量检测与临床的关系

目的：探讨血清HBV DNA水平与HBV标志（HBV M）表现模式，肝功能状态，肝内炎症的关系。方法：对219例排除甲，丙，丁和戊型肝炎病毒的混合与重叠感染患者中的HBsAg阳性的慢性乙型肝炎患者进行肝穿刺病理检查。HBV DNA定量采用荧光定量PCR分析系统，HBV M采用ELISA法。结果：血清HBV DNA水平与HBVM表现模式有关，HBsAg与HBeAg的存在影响HBV DNA水平变化。在HBsAg阳性患者中，HBV DNA水平与Scheuer分级无明显相关。血清谷丙转氨酶水平与HBV DNA水平无明显相关。结论HBeAg和HBV DNA有明显的相关，抗－HBe阳性者病毒未完全停止复制，只是复制水平降低。单抗HBe阳性，单抗HBc阳性，抗HBs阳性和抗HBc阳性未检出HBV DNA，肝内炎症活动程度与血清HBV DNA水平无明显关系。

Nucleic acid quantifying assay of hepatitis B virus

Objective To explore the relation between the nucleic acid content of hepatitis B virus and hepatitis B virus markers, liver function and its pathological state. Methods Thirty-nine patients suffering from chronic hepatitis B with positive HBsAg were performed liver puncture for histopathological examination. Fluorescence ration PCR analysis was used for HBV DNA quantifying assessment. Hepatitis B virus markers were detected by ELISA. Results Serum HBV DNA content was related to hepatitis B virus markers. There was no correlation between HBV DNA level and the Scheuer grade in patients with positive HBsAg, and no obvious correlation between serum glutamic pyruvic transaminase level and HBV DNA content. Conclusions There is a significant correlation between HBeAg and HBV DNA. but not between liver inflammation stage and serum HBV DNA content.