基质金属蛋白酶-2在侵袭性垂体腺瘤中的表达

目的　探讨基质金属蛋白酶 2 (matrixmetalloproteinase 2 ,MMP 2 )的表达与侵袭性垂体腺瘤的关系。方法　用免疫组化的方法对 5 4例垂体瘤患者组织标本中MMP 2的表达进行检测。并对其中 16例患者采用逆转录 聚合酶链反应 (RT PCR)的方法检测了MMP 2mRNA的表达。免疫组化结果用半定量的方法进行分析。结果　 5 4例垂体瘤患者中 ,有 32例女性 ,2 2例男性。其中 ,12例为侵袭性垂体腺瘤 ,4 2例为非侵袭性垂体腺瘤。免疫组化显示 ,侵袭性垂体腺瘤的MMP 2的表达明显高于非侵袭性垂体腺瘤 (分别为 4 .3± 0 .5 ;2 .3± 0 .2 ;P <0 .0 1)。大型垂体腺瘤和微垂体腺瘤 ,以及功能性和非功能性垂体腺瘤的MMP 2的表达则无明显差别。MMP 2的表达与Ki 6 7的表达无明显相关性 (r =$C 0 .0 5 ,P >0 .0 5 )。侵袭性垂体腺瘤MMP 2mRNA的表达 ,明显高于非侵袭性垂体腺瘤 (6 8.3± 15 .3;2 1.8± 8.2 ;P <0 .0 5 )。结论　MMP 2的高表达与肿瘤的侵袭性密切相关。但与肿瘤的大小及分泌功能无明显关系。MMP 2可以作为肿瘤侵袭性一项有效的指标

Matrix metalloproteinase 2 expression in invasive pituitary adenomas

Objective To evaluate the relationship between the expression of matrix metalloproteinase 2(MMP-2) and the cavernous invasion of pituitary adenomas. Methods The expression of MMP-2 protein in tissue samples from 54 pituitary adenomas was examined by immunohistochemical method. In sixteen patients, the expression of MMP-2 mRNA was also examined by RT-PCR assay. Immunostaining was semiquantatively scored based on intensity (0-3) and distribution (0-3). Results Thirty-four patients were femal and 20 were male.Twelve patients showed cavernous invasion, and 42 noninvasion. The MMP-2 score of pituitary adenoma patients with cavernous invasion (4. 3 ±0. 5 ) was significantly higher than that without invasion (2. 3 ± 0. 2 ). No correlation was observed between macroadenomas and microadenomas, and also, no correlation between the functional adenomas and non-functional adenomas. Positive correlation was not found between the MMP-2 and Ki67 labeling index ( r = -0.05, P ＞ 0.05 ). MMP-2 mRNA expression was also observed significantly higher in invasive pituitary adenomas (68.3 ± 15.3 ) than those without invasion ( 21.8 ± 8.2, P ＜ 0. 05 ). Conclusion MMP-2 may be associated with the aggressiveness and invasion of pituitary adenomas and is not related with the tumor size and secretion. MMP-2 is a useful tool for assessing the invasion of pituitary adenomas.