三丫苦对高脂饮食性胰岛素抵抗模型大鼠GLP-1mRNA的影响

目的:观察三丫苦对高脂饮食性胰岛素抵抗大鼠GLP-1mRNA的影响。方法:40只SD雄性大鼠随机分为正常对照组、模型对照组、三丫苦组、罗格列酮组,建立高脂饮食性胰岛素抵抗大鼠模型。检测各组大鼠TC、TG、FBG、FINS等指标的变化,清醒状态下高胰岛素-正葡萄糖钳夹实验检测葡萄糖输注率,Real-time PCR法检测肝脏组织GLP-1mRNA的表达。结果:与正常对照组比较,模型对照组TC、TG、FBG、FINS升高(P0.01),葡萄糖输注率降低(P0.01)。给予三丫苦后,大鼠TC、TG、FBG、FINS降低(P0.01或P0.05),GIR水平升高(P0.05),与罗格列酮组比较TC和TG降低(P0.05)。与正常对照组比较,模型对照组GLP-1mRNA表达显著降低(P0.01)。与模型对照组比较,三丫苦组和罗格列酮组GLP-1mRNA表达均有不同程度的升高(P0.01或P0.05)。与罗格列酮比较,三丫苦组GLP-1mRNA表达升高(P0.05)。结论:三丫苦对高脂饮食性胰岛素抵抗大鼠糖脂代谢及GLP-1mRNA有一定调节作用。

Effect of Sanyaku on GLP-1mRNA of Insulin-resistance Rats Induced by High-fat Diet

Objective:To observe the effect of Sanyaku on GLP-1mRNA of insulin-resistance rats induced by high-fat diet.Method:40 male SD rats were divided into 4 groups(normal control group,model control group,Sanyaku group and Rosiglitazone group) randomly.The insulin-resistance rat models induced by high-fat diet were established.The change of the index TC,TG,FBG,FINS of each group was examined.The glucose infusion rate(GIR) was examined by hyperinsulinemic-euglycemic clamp when the rats were in consciousness.The expression of GLP-1mRNA in liver tissue were detected by Real-time PCR method.Result:The TC,TG,FBG and FINS were obviously higher and the GIR were obviously lower in the model control group than in the normal control group(P<0.01).After given Sanyaku,the rats’ TC,TG,FBG and FINS decreased(P<0.01 or P<0.05) and the GIR increased(P<0.05).Compared with TC and TG of the Rosiglitazone group,that of the Sanyaku group decreased(P<0.05).Compared with the expression of GLP-1mRNA of the normal control group,that of the model control group decreased obviously(P<0.01).Compared with the expression of GLP-1mRNA of the model control group,that of the Sanyaku group and the Rosiglitazone group increased at different degrees(P<0.01 or P<0.05).Compared with the expression of GLP-1mRNA of the Rosiglitazone group,that of the Sanyaku group increased(P<0.05).Conclusion:Sanyaku has regulating effect on glucose and lipid metabolism and GLP-1mRNA of insulin-resistance rats induced by high-fat diet.