MBL免疫治疗结核病小鼠的实验研究

目的观察甘露聚糖结合凝集素(mannan-binding lectin,MBL)对结核分枝杆菌感染小鼠的免疫治疗作用。方法将BALB/c小鼠18只随机分成MBL免疫治疗组、微卡治疗组和结核菌感染对照组,每组6只。小鼠感染结核分枝杆菌后第14、21、28 d各组腹腔内分别注射MBL、微卡和生理盐水。治疗结束后第3周处死小鼠,观测各组小鼠体质量以及肺、脾脏湿重,观察肺脏病理改变,取肺、脾组织进行结核菌培养和菌落计数。结果治疗后第3周,治疗组小鼠体质量和脾脏质量高于对照组,肺脏质量低于对照组,且肺部病变较轻。MBL减轻结核病小鼠体质量下降,减少结核病小鼠肺和脾脏结核菌数量。结论MBL对结核病小鼠具有一定的免疫治疗作用。

Immunotherapeutic effects of mannan-binding lectin on murine with pulmonary tuberculosis

Objective To study the immunotherapeutic effects of MBL(mannan-binding lectin) on murine infected with Mycobacterium tuberculosis.Methods Eighteen BALB/ c mice were randomized into 3 groups,One group treated with mannan-binding lectin,another group treated with vaccae vaccine,The third group treated with PBS(n=6).1×10⁶ bacili in a volume of 300 μL PBS were injected into the tail vein of mice in each group.The mice from each group were treated intraperitoneally with MBL,Vaccae(freeze-dried M.vaccae vaccine for therapy) and PBS at 2 weeks postinfection,respectively.The animals were sacrificed at 2 weeks after treatment.The weights of mice,lungs and spleens were measured.Then the lungs and spleens of mice were processed for histological examination.The numbers of viable bacteria in lungs and spleens were counted.Results At 2 weeks after therapy,the body weights of mice in treatment groups were higher than that in control group,the lung weights of mice in treatment groups were lower than that in control group,the spleen weights of mice in treatment groups were higher than that in control group.The numbers of viable bacteria in lungs and spleens of mice from treatment groups were lower than that in control group,respectively.MBL could improve host defense against Mycobacterium tuberculosis and reduce mycobacteria outgrowth on murine with tuberculosis.Conclusion MBL had immunotherapeutic effects on murine with tuberculosis.