一氧化氮在梗阻性黄疸大鼠肝、肾、肠组织中含量变化及意义

目的 了解一氧化氮（NO）在梗阻性黄疸大鼠肝、肾、肠组织中含量变化及意义。方法 大鼠胆总管结扎后,分别于第一周内和第三周内应用Aminoguanidine（AG）抑制NO合成,同时应用生理盐水（NS）作对照,检测不同时段抑制NO合成后大鼠肝、肾、肠组织中NO和丙二醛（MDA）含量、肌酐清除率（Ccr）血清总胆红素（T-BIL）和丙氨酸氨基转移酶（ALT）含量及肠系膜淋巴结细菌移位（BT）率的变化。结果 胆总管结扎后,大鼠肝、肾、肠组织中NO含量明显升高,在胆总管结扎第一周抑制NO合成后,肝、肾、肠组织中NO含量明显下降,MDA含量明显升高,血ALT明显升高、Ccr明显下降、肠系膜淋巴结BT率明显升高;而在胆总管结扎第三周抑制NO合成后,肝、肾、肠组织中NO和MDA含量明显下降,血ALT明显下降、Ccr明显升高。肠系膜淋巴结BT率明显下降。结论NO在胆道梗阻引起的肝、肾、肠粘膜屏障功能障碍的发生机制中具有重要作用,既有保护作用,又有损害作用。梗阻早期表现为对组织的保护作用,后期表现为对组织的损害作用。

Changes of concentration and effect of nitric oxide in liver, kidney, intestine of rats with obstructive jaundice

Objective To observe the concentration and effect of nitric oxide in liver, kidney, intestine of rats with obstructive jaundice rats. Methods Changes of the concentration of nitric oxide (NO) and malondialdehyde (MDA) in liver, kidney, intestine were measured, creatinine clearance, the concentration of total bilirubin (T- BIL) and ALT in plasma and the incidence of bacterial translocation (BT) in mesenteric lymph nodes were observed at the end of the first and the third week following common bile duct ligation with a week - dose aminoguanidine (AG) or normal saline (NS) before sampling. Results The concentration of NO was significantly increased in liver, kidney, intestine after common bile duct ligation in rats. at the first week, the concentration of MDA in liver kidney, intestine was significantly increased, creatinine clearance was significantly reduced, plasma ALT was significantly increased, the incidence of BT in mesenteric lymph nodes was significantly increased after inhibiting NO synthesis with AG, but at the third week, the result was completely opposite after inhibiting NO synthesis with AG. Conclusions NO has dual roles in impairment of liver, kidney, intestine in obstructive jaundice,which is advantageous at the first week and is harmful at the third week.