VWA、FGA、PENTAE三个STR基因座在内蒙古地区土左旗蒙古族人群中的遗传多态性研究

目的：研究内蒙古土左旗蒙古族群体VWA、FGA、PENTAE基因多态性。方法：应用多重PCR技术及聚丙烯酰胺凝胶垂直板电泳分型。结果：首次获得生活在农区的蒙古族人群三个STR基因型分布频率，VWA发现8个等位基因，FGA发现14个等位基因，PENTAE发现21个等位基因。3个基因座的基因型频率分布均符合Hardy-Weinberg平衡(P＞0．05)。各基因座的杂合度分别为：0．7945、0．8486和0．9234，非父排除率分别为：0．5919、0．6962和0．8409，个人识别能力分别为：0．9160、0．9536和0．9826，多态性信息总量分别为：0．7617、0．8293和0．9161。结论：3个基因座在内蒙古地区土左旗蒙古族群体中具有较高的非父排除率和个人识别几率，在法医学应用和群体遗传学研究中具有较高的价值。

GENETIC POLYMORPHISM OF VWA,FGA,PENTAE LOCI IN TUZUOQI MONGOLIAN GROUP

Objective: To evaluate population polymorphisms of the VWA?FGA and PENTAE loci in Tuzuoqi Mongolian group of Inner Mongolia. Methods: The multiplex polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis and silver staining were used. Results: Allele frequencies of the three loci were first calculated from Mongolian nationality population in Tuzuoqi in Mongolia. Eight alleles were found at VWA locus, fourteen alleles at FGA locus and twenty|one alleles at PENTAE locus. No deviations from Hardy|Weinberg equilibrium were observed. The heterozygosities observed were 0.7945, 0.8486 and 0.9234 for VWA?FGA and PENTAE respectively. The chances of exclusion were 0.5919, 0.6962 and 0.8409,the discrimination powers were 0.9160, 0.9536 and 0.9826 and the polymorphic information content were 0.7617, 0.8293 and 0.9161. Conclusion: All of the three loci in this study were useful markers for individual identification and paternity test and there are good value in forensic and population genetics research.;