HPLC法同时测定抗骨增生片中麦角甾苷﹑柚皮苷和淫羊藿苷的含量

目的建立同时测定抗骨增生片中麦角甾苷﹑柚皮苷和淫羊藿苷含量的方法。方法采用HPLC法。色谱柱:Diamonsil C18(4.6 mm×200 mm,5μm),流动相:乙腈-体积分数为0.08%的磷酸水溶液-四氢呋喃(体积比为10∶90∶22),流速:1.0 mL·min-1,检测波长:270 nm,柱温:35℃。结果麦角甾苷质量浓度在26.0～260.0 mg·L-1内、柚皮苷质量浓度在3.0～30.0 mg·L-1内、淫羊藿苷质量浓度在4.3～43.0 mg·L-1内与峰面积呈良好的线性关系,相关系数分别为0.999 80、.999 7和0.999 9,平均回收率分别为98.5%﹑98.8%和99.2%。结论本方法可作为抗骨增生片的质量控制方法之一。

Simultaneous determination of the contents of acteoside,naringin and icariine in Kangguzengsheng tablets by HPLC

Objective To establish an HPLC method for simultaneous determination of acteoside, naringin and icariine in Kangguzengsheng (traditional Chinese medicine) tablets. Methods The chromatographic separation was carried out on a Diamonsil C₁₈ column (200 mm×4.6 mm, 5 μm) with acetonitrile-0.08% phosphoric acid-tetrahydrofuran(V∶V∶V=10:90:22) as the mobile phase. The flow rate was 1.0 mL·min^-1 and column temperature was 35 ℃.The detection was set at the wavelength of 270 nm. Results The calibration curve was linear within the range of 26.0-260.0 mg·L^-1(r=0.999 8), 3.0-30.0 mg·L^-1(r=0.999 7), 4.3-43.0 mg·L^-1(r=0.999 9), for acteoside, naringin and icariine, respectively. The average recoveries were 98.5% ,98.8% and 99.2% for acteoside, naringin and icariine, respectively. Conclusions The method is simple, accurate and reproducible, which can be used for the quality control of Kangguzengsheng tablets.