复方甘草甜素下调细胞周期素依赖性激酶1启动子表达

目的:了解复方甘草甜素调节细胞周期素依赖性激酶 1(CDK1)基因启动子的活性,为甘草甜素的作用机制提供理论依据． pCAT3-basic空载体的12．4倍,是甘草甜素刺激转染pCAT3- CDK1-P的HepG2细胞的9．3倍．结论:复方甘草甜素可以下调CDK1基因启动子的活性,进而下调CDK1基因的表达,为深入了解甘草甜素在肝纤维化和肝癌中的作用机制提供新的分子生物学依据．

Down-regulatory effects of glycyrrhizin on expression of cyclin-dependent kinase 1 gene promoter

AIM: To investigate the regulatory effects of the glycyrrhizin on the expression of cyclin-dependent kinase 1 (CDK1) gene promoter as well as its molecular biological mechanisms. METHODS: The report gene expression vector pCAT3-CDK1-P was transfected into the HepG2 cell line, and then stimulated with glycyrrhizin. The HepG2 cells transfected with pCAT3-basic was used as a negative control. The expression level of chloramphenicol acetyltransferase (CAT) in the transfected HepG2 cells was detected by enzyme-linked immunoassay (ELISA) after 24 h. RESULTS: pCAT3-CDK1-P activated the expression of CAT in HepG2 cells, while the glycyrrhizin inhibited it. The activity of CAT in pCAT3-CDK1-P transfected cells without stimulation was 9.3 times as high as that in pCAT3-CDK1-P transfected cells stimulated with glycyrrhizin, and 12.4 times as high as that in pCAT3-basic transfected cells. CONCLUSION: Glycyrrhizin can down-regulate the expression of CDK1 gene promoter, and further down-regulate the expression of CDK1 gene.