HBV宫内感染儿童接种乙型肝炎疫苗免疫失败与IL-2及其受体的关系

目的从ＩＬ－２及其受体角度探讨宫内感染ＨＢＶ导致乙型肝炎（乙肝）疫苗免疫失败的机制。方法采用体外细胞培养、流式细胞仪和酶联免疫检测技术对２２例ＨＢＶ宫内感染儿童和１５例正常儿童的外周血单个核淋巴细胞（ＰＢＭＣ）在非特异性刺激（ＰＨＡ）和特异性刺激（ＨＢｓＡｇ）下的膜ＩＬ－２受体表达水平和ＩＬ－２含量进行了测定。结果在ＨＢｓＡｇ刺激情况下，ＨＢＶ宫内感染儿童接种乙肝疫苗无反应组ＰＢＭＣ的ＩＬ－２膜受体表达水平低于有反应组和正常儿童组。在ＰＨＡ或ＨＢｓＡｇ刺激情况下，ＨＢＶ宫内感染儿童接种乙肝疫苗无反应组ＰＢＭＣ细胞培养上清的ＩＬ－２水平低于有反应组和正常儿童组。结论ＩＬ－２分泌能力低下，以及ＩＬ－２膜受体表达不足可能是宫内感染ＨＢＶ导致乙肝疫苗免疫失败的机制之一。

Study on the relationship between IL 2/IL 2R and immunization failure of hepatitis B vaccine in the children with HBV intrauterine infection

Objective To study the mechanism of immunization failure of Hepatitis B vaccine by analyzing the changes of IL 2/IL 2R system in HBV intrauterine infection children. Methods Peripheral blood mononuclear cells (PBMC) isolated from 22 intrauterine HBV infected children and 15 healthy children were incubated in the presence of PHA as well as purified HBsAg.Membrane IL 2R (mIL 2R) expression of activated cells was determined by using Flow Cytometry Analysis System (FCAS).The levels of Interleukin 2 in the supernatants of activated cells were determined by Enzyme Linked Immunoassay. Results The extent of expression of mIL 2R in PBMC stimulated by HBsAg as well as the level of IL 2 in the supernatants of PHA or HBsAg activated PBMC cultures in nonresponders of HBV intrauterine infection children was lower than that of responders and normal controls. Conclusion Low level of IL 2 production and IL 2R expression under the HBsAg stimulation may contribute to the failure of immunization in HBV infected children.