乳腺癌中COX-2、Bax、Bcl-2的表达及熊果酸的干预作用

目的研究熊果酸（UA）对人乳腺癌SK-BR-3细胞COX-2、Bax、Bcl-2 mRNA的表达的影响,结合人体乳腺癌组织凋亡相关蛋白表达检测,探讨其作用机制,为UA的临床应用提供实验依据。方法 RT-PCR技术检测SK-BR-3细胞中COX-2、Bax、Bcl-2 mRNA的表达;免疫组织化学技术检测雌激素受体（ER）（-）,人表皮生长因子受体-2（HER-2）（＋）的人乳腺癌组织中COX-2、Bax、Bcl-2蛋白的表达。结果 UA作用48 h后能使乳腺癌SK-BR-3细胞中COX-2、Bcl-2 mRNA表达明显减少（P〈0.05）,使Bcl-2/Bax比值减小（P〈0.05）,而对Bax mRNA的表达未见明显影响;COX-2、Bcl-2、Bax蛋白在ER（-）,HER-2（＋）的乳腺癌组织中的表达阳性率分别为86.67%（26/30）,63.33%（19/30）,56.67%（17/30）,COX-2与Bax表达呈明显负相关（P〈0.05）,与Bcl-2未见明显相关性。结论 UA可抑制COX-2、Bcl-2 mRNA表达,使Bcl-2/Bax减小;ER（-）,HER-2（＋）的人乳腺癌组织中COX-2中阳性表达率较高,COX-2与Bax表达呈明显负相关,与Bcl-2表达未见明显相关性。

The expressions of COX-2, Bax and Bcl-2 in the tissues of breast cancer and intervention effect of ursolic acid

Objective To investigate the expressions of COX-2,Bcl-2 and Bax mRNA in human breast cancer SK-BR-3 cells after incubation with UA and to dedect the expression of apoptosis-related proteins in the tissue of breast cancer,and to provide a reliable experimental basis for clinical applicaton of UA.Methods The expressions of COX-2,Bcl-2 and Bax mRNA in SK-BR-3 cells were determined by RT-PCR;The expressions of COX-2,Bcl-2 and Bax protein in estrogen receptor（ER）-negative and human epidermal growth-factor 2（HER2） positivity human breast cancer were determined by immunohistochemistry.Results The expression of COX-2 and Bcl-2 mRNA of SK-BR-3 cells treated by UA for 48 h decreased（P〈0.05）,Bcl-2 / Bax reduced（P〈0.05）,whereas the expression of Bax mRNA unchanged;The positivvity rate of COX-2、Bcl-2、Bax protein in estrogen receptor-negative and human epidermal growth-factor 2 positive breast cancer were 86.67%（26/30）,63.33%（19/30） and 56.67%（17/30）,respectively.The expression of COX-2 and Bax was negatively correlated（P〈0.05）,whereas the expression of COX-2 and Bcl-2 was no significant correlation.Conclusion UA inhibites COX-2 and Bcl-2 mRNA expression and reduces the ratio of Bcl-2 / Bax;There is a higher positive expression of COX-2 in estrogen receptor-negative and human epidermal growth-factor 2 positive human breast cancer;The expression of COX-2 and Bax is negatively correlated,whereas the expression of COX-2 and Bcl-2 is no significant correlation.