| HPLC法测定龙芽梅木药材中齐墩果酸的含量 |
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| 引用本文： | 谭朝阳,袁宏佳. HPLC法测定龙芽梅木药材中齐墩果酸的含量[J]. 中国中医药信息杂志, 2010, 17(1): 46-47 |
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| 作者姓名： | 谭朝阳 袁宏佳 |
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| 作者单位： | 湖南中医药大学药学院,湖南,长沙,410208 |
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| 摘 要： | 目的建立HPLC法测定龙芽梅木药材中齐墩果酸的含量测定方法。方法采用Kromasil C18色谱柱（250mm×4．6mm，5μm），流动相：乙腈-水-磷酸（80：20：0．42），检测波长：208nm。结果齐墩果酸在1．164～5．820邮范围内具有良好的线性关系，r=0．9999，平均加样回收率为99．69％，RSD=1.76％（n=5）。结论该方法简单、准确，重复性好，可作为龙芽橡木药材中齐墩果酸的含量测定方法。
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| 关 键 词： | 龙芽橡木 齐墩果酸 高效液相色谱法 含量测定 |
Determination of Oleanolic Acid in Cortex Aralia Elatae by HPLC |
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| TAN Zhao-yang,YUAN Hong-jia. Determination of Oleanolic Acid in Cortex Aralia Elatae by HPLC[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2010, 17(1): 46-47 |
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| Authors: | TAN Zhao-yang YUAN Hong-jia |
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| Affiliation: | TAN Zhao-yang,YUAN Hong-jia(Pharmacy College,Hunan University of TCM,Changsha 410208,China) |
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| Abstract: | Objective To establish a method for determination ofoleano/ic acid in Cortex Aralia Elatae by HPLC. Methods The determination was carried out with Kromasil C18 column （250 min×4.6 mm, 5 μm）, using acetonitrile-water-phosphoric acid （80 ： 20 ： 0.421 as the mobile phase and detected at the wavelength of 208 nm. Results Oleanolic acid showed a good linear relationship at the range of 1.164-5.820 μg, r = 0.9999. The average recovery was 99.70% and RSD was 1.76% （n =5）. Conclusion The method was simple and accurate with good reproducibility, and can be used to determine the content of oleanolic acid in Cortex Aralia Elatae. |
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| Keywords: | Cortex Aralia Elatae oleanolic acid HPLC content determination |
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