hnRNP B1特异性小干扰RNA对肺癌细胞及p53表达影响的研究进展

核内不均一核糖核蛋白(hnRNP)B1的高表达是肺癌频发早期事件,可能与细胞分化失调控有关. p53是目前公认的一种抑癌基因,当细胞受到各种信号刺激引起基因应激反应时,p53蛋白被磷酸化而变构从而被活化,抑制细胞的分裂、增殖和诱导细胞凋亡,防止细胞的恶性转化.应用RNA干扰技术中的小干扰RNA使hnRNP B1基因沉默,能使p53基因的活性增强,从而抑制细胞的恶性转化.本文就hnRNP B1特异性小干扰RNA对肺癌细胞p53基因表达影响的研究进展作一综述.

Research progress of effects of hnRNP B1 specific small interfering RNA on lung cancer cells and p53 expression

Overexprssion of heterogeneous nuclear ribonucleoprotein (hnRNP) Bl is a frequent early event in lung cancer,may be related to maladjustment of cell differentiation. p53 is widely recognized as a tumor suppressor gene at present. When the cells are stimulated by various signals, which induces gene stress reaction, p53 protein is activated by phosphorylation and allosterism, which inhibits cell division,proliferation and induces apoptosis to prevent malignant transformation of cells. Application of small interfering RNA in RNA interference technology silences hnRNPBl gene,enhances the activation of p53 gene, thus inhibits the malignant transformation of cells. This article reviews the research progress of effects of hnRNPBl specific small interfering RNA on lung cancer cells and p53 gene expression.