| 冷应激对大鼠脑组织cAMP和cGMP水平及AC和PDE生物活力的影响 |
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| 引用本文： | 杨国平,王洪军,张锐,杨姝明,孙艳.冷应激对大鼠脑组织cAMP和cGMP水平及AC和PDE生物活力的影响[J].工业卫生与职业病,2009,35(2):77-80. |
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| 作者姓名： | 杨国平 王洪军 张锐 杨姝明 孙艳 |
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| 作者单位： | 沈阳军区联勤部疾病预防控制中心,辽宁,沈阳,110034;沈阳工业安装工程南京分公司,江苏,南京,210029;沈阳药科大学,辽宁,沈阳,110016 |
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| 摘 要： | 目的通过对冷应激大鼠脑组织环磷酸腺苷(cAMP)与环磷酸鸟苷(cGMP)含量,腺苷酸环化酶(AC)和磷酸二酯酶(PDE)生物活力的研究,旨在探讨冷应激对中枢神经系统损伤的机制。方法将大鼠分对照组与实验组〔含冷应激组和冷应激恢复组(以下简称恢复组)〕,cAMP、cGMP含量检测采用酶联免疫分析法(ELISA)。AC和PDE的生物活力检测采用放射性同位素法。结果大鼠脑皮质cAMP含量和AC活力:对照组为(0.516±0.271)pmol/mg和(1.67±0.52)pmol/(mg.min),冷应激组为(0.338±0.204)pmol/mg和(1.38±0.15pmol/(mg.min),而恢复组cAMP含量为(0.419±0.197)pmol/mg,与对照组比较,差异有统计学意义(P<0.05)。大鼠脑干cAMP含量和AC活力:对照组为(0.326±0.212)pmol/mg和(2.21±0.13)pmol/(mg.min),冷应激组为(0.297±0.224)pmol/mg和(1.95±0.14)pmol/(mg.min),与对照组比较,差异有统计学意义(P<0.05)。大鼠丘脑下部cAMP含量和AC活力:...
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| 关 键 词： | 冷应激 环磷酸腺苷 腺苷酸环化酶 磷酸二酯酶 |
Effect of Cold Stress on Levels of cAMP and cGMP and Bioactivities of AC and PDE in Brain Tissues of Rat |
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| YANG Guo-ping,WANG Hong-jun,ZHANG Rui,YANG Shu-ming,SUN Yan.Effect of Cold Stress on Levels of cAMP and cGMP and Bioactivities of AC and PDE in Brain Tissues of Rat[J].Industrial Health and Occupational Diseases,2009,35(2):77-80. |
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| Authors: | YANG Guo-ping WANG Hong-jun ZHANG Rui YANG Shu-ming SUN Yan |
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| Institution: | * Centre of Disease Prevent and Control of Shenyang Command;Shenyang 110034 China |
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| Abstract: | Objective Through the study on the effect of cold stress on levels of cAMP(cyclic adenosine mono-phosphate)and cGMP(cyclic guanosine mono-phosphate)and bioactivities of AC(adenylyl cyclase)and PDE(phosphodiesterase)in brain tissues of rat to explore the impairment mechanism of central nerve system induced by cold stress.Methods Wistar rats were divided into the control group,cold stress group and recovery group.The cAMP and cGMP contents were determined by enzyme linked immunosorbent assay.Radioisotope meth... |
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| Keywords: | Cold stress Cyclic adenosine mono-phosphate Adenylyl cyclase Phospho-diesterase |
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