Utility of CD127 combined with FOXP3 for identification of operational tolerance after liver transplantation |
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| Institution: | 1. Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut 71515, Egypt;2. Department of Hematology and Immunology, College of Medicine, Umm Al-Qura University, Mecca 50431, Saudi Arabia;3. Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan;4. Department of Anesthesia, Faculty of Medicine, Assiut University, Assiut 71515, Egypt;5. Department of Anesthesiology, King Abdullah Medical City, Mecca 21955, Saudi Arabia;6. World Premier International Research Center, Immunology Frontier Research Center, Osaka University, Osaka 565-0871, Japan;7. Transplantation Laboratory, The University of Sydney, Sydney, NSW 2006, Australia;8. Department of Disaster and Comprehensive Medicine, Fukushima Medical University, Fukushima 960-1295, Japan;1. Charité-University-Medicine Berlin, Campus Rudolf Virchow, Department of Cardiology, Berlin, Germany;2. Berlin–Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany;3. DZHK (German Center for Cardiovascular Research), partner site Berlin, Germany;4. Charité-University-Medicine Berlin, Campus Benjamin Franklin, Department of Cardiology, Berlin, German;5. Heart and Vascular Center (HGZ), Bad Bevensen, Germany;6. Medical College of Wisconsin, Milwaukee, USA;7. Department of Cardiovascular Physiology, Institute of Physiology, Ruhr University Bochum, Bochum, Germany;8. Department of Cardiology, Deutsches Herzzentrum Berlin (DHZB), Berlin, Germany;1. Department of Ophthalmology, The 2nd Hospital of Shandong University, Jinan 250031, China;2. Department of Ophthalmology, General Hospital of Liaohe Oil Field, Panjin 124000, China;3. The General Hospital of PLA, Beijing 100853, China |
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| Abstract: | Loss of cell surface expression of CD127 on CD4+ CD25++ regulatory T-cells (Tregs) may be a useful marker to efficiently isolate Tregs. As FOXP3 was specifically used to identify Tregs, combining these two markers could give better identification for patient with operational tolerance (OT) after liver transplantation. To testify this mixed lymphocyte reaction (MLR), the function of circulating CD4+ CD25++ CD127dim cells (CD127dim cells) was examined in immunosuppression (IS)-free pediatric recipients after liver transplantation (LTx) (group operational tolerance: OT) (Gr-tol n = 25) compared to recipients who could not stop IS due to clinically overt rejection (group intolerance) (Gr-intol n = 18), recipients who were weaning IS (Gr-weaning n = 11) and age-matched healthy volunteers (Gr-vol n = 11). In addition, the frequencies of CD127dim cells vs CD4+ CD25++ CD127dimFOXP3+ (CD127dimFOXP3+) cells were compared in these four groups by FACS analyses. Our results showed that The proliferation of CD4 cells to donor antigens was reduced compared to third-party antigens only in Gr-tol (P = 0.022) but not in other groups (P = NS). Depletion of CD127dim cells resulted in a donor antigen-specific abrogation of this MLR hyporesponsiveness in Gr-tol (P < 0.001) but not other groups (P = NS). This implied that CD127 efficiently isolated donor antigen-specific Tregs. The frequencies of CD127dim cells were significantly lower in Gr-intol (5.2% ± 1.9%) compared to those in Gr-tol (7.8% ± 1.8%) (P < 0.001) as were the frequencies of CD127dim FOXP3+ cells (Gr-tol: 5.4% ± 1.7% vs Gr-intol: 2.9% ± 1.0%, P < 0.001). Of interest, there were fewer CD127dimFOXP3+ cells in Gr-intol (2.9% ± 1%) than in Gr-weaning (5.1% ± 1.8%) (P = 0.002), but no difference in CD127dim cells (Gr-intol: 5.2% ± 1.9% vs Gr-weaning: 6.7% ± 2.0%) (NS). Thus, combining FOXP3 with CD127 for phenotype analysis demonstrated an unequivocal difference between Gr-intol and Gr-weaning that was not detected by CD127 alone. In conclusion CD127 was a useful surface marker to isolate donor-antigen-specific-Tregs in OT after LTx. The additive effect of its combination with FOXP3 is important in phenotypical Treg analyses of OT patients. |
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