p14^ARF和p53蛋白表达与脑胶质瘤发生发展的相关性研究

目的：探讨脑胶质瘤中p14^ARF和p53蛋白表达与不同病理级别脑胶质瘤的相关性及两者之间的相互作用。方法：应用免疫组织化学技术检测31例不同病理分级脑胶质瘤中的上述遗传学改变，5例脑挫裂伤脑组织为正常对照。结果：p14^ARF和p53蛋白在低级别（Ⅰ，Ⅱ级）和高级别（Ⅲ，Ⅳ级）脑胶质瘤中的表达阳性率分别为75．00％和30．43％，12．50％和69．57％。两蛋白在低级别和高级别中的表达差异均有显著性（P〈0.05）。p14^ARF表达水平与脑胶质瘤病理分级之间呈显著负相关性（r=0．571，P〈0．01），即随着肿瘤恶性度的升高而降低。而突变型p53蛋白正相反，与病理分级呈显著正相关性（r=0．472，P≤0．01），即随着肿瘤恶性度增高而增高。p14^ARF和p53蛋白之间在肿瘤中存在明显的相互作用（P〈0．05）。结论：p14^ARF和p53蛋白表达异常可导致细胞周期调控失常和细胞异常增殖，并且二者之间存在协同作用，可加速脑胶质瘤恶性演进。

Study on the expression and relationship of p14^ARF protein and p53 protein in human glioma

Oblectlve：To detect expression oi p14^ARF protein,p53 protem and explore three proteins＇ relationship in the genesis and development of human glioma in different grade gliomas. Methods：Expression of p14^ARF protein,p53 protein were detected by using S-P immunohistochemical technique in 31 cases of brain glioma with different pathological types and 5 cases of normal brain tissue. Results ：The expression rate of p14^ARF protein,p53 protein in low and high pathological grades was 75.00% and 30. 43% （P〈0. 05） ,12. 50% and 69. 57% （P〈0. 05）. A significant negative eorelation （r= -0. 571 ,P〈0.01） was observed between the p14^ARF protein and pathological grades. On the contrary, mutant p53 protein showed a positive corelation with pathological grades （r= 0. 472 ,P≤0.01）. The expression levels of p14^ARF protein was higher with lower grade of malignancy. But the expression lever of p53 protein increased parallel with the elevation of glioma malignant degree. And there was a significant eorelationship between the two kinds of proteins （P〈0.05）. Conclusion：This study suggests that the expression of p14^ARF protein and p53 protein play very important roles in the genesis and development of human brain glioma and they may coexist in it. The abnormal expression of the two proteins cause disorder of cell cycle modulation and increase of cellular proliferation activity which is an important factor to influence the biological character of brain glioma.