自体骨髓基质细胞移植治疗兔膝关节半月板无血运区损伤

目的 观察自体骨髓基质细胞在体内分化为软骨细胞的能力及对半月板无血运区损伤修复的影响。方法 将体外培养的自体骨髓基质细胞收集后与纤维蛋白凝胶混合,植入４０只成年兔一侧膝半月板无血运区损伤的裂口内,另侧设空白对照或单纯纤维蛋白凝胶植放。分别于术后１（１２只）、２（１２只）、３（１５只）个月取村,行肉眼、光镜组织学观察。结果 术后２个月１２个膝半月板损伤处在量的软骨细胞并有胶原纤维形成,３个月１３个膝

Bone marrow stromal cells autograft in the treatment of knee joint meniscal injury in the avascular zone

Objective\ To observe the ability of bone marrow stromal cells transform into chondrocyte and the influence of bone marrow stromal cell autograft on meniscal injury in avascular zone. Methods Forty adult rabbits' bone marrow stromal cells were cultured and passaged. Then the cells were harvested and mixed with fibrin glue. The mixture was implanted into the wound of meniscal injury in avascular zone in one knee of 40 rabbits. Control group and single fibrin glue implant group were set up in another knee. The tissue specimens obtained after one month, two months and three months were observed grossly and under light microscope respectively. Results\ A great number of chondrocytes were seen in the wound of meniscal injury and collagenous fibers were formed two months after operation in 12 specimens, then the wound of meniscal injury in avascular zone healed with fibrocartilage-like tissue three months after operation in 13 specimens in experimental group. In contrast, the wound of meniscal injury did not heal in all contral groups and healed with scar-like tissue in single fibrin glue implant group. Conclusion\ Bone marrow stromal cells maybe transform into chondrocyte and promote the healing of meniscal injuries in avascular zone inside knee joint cavity.