低强度He-Ne激光照射对成纤维细胞功能的调节作用

目的研究低强度He-Ne激光对人正常皮肤成纤维细胞增殖及胶原合成功能的调节作用。方法采取固定照射时间、选用不同激光照射强度的方法来研究激光生物凋节效膻与照射剂量问的关系。采用MTT法[3-（4，5-Dimethylthiazol-2-yl）-2，5-diphenylIeIrazolium bromide]和羟脯氨酸试剂分别检测不同强度激光照射对细胞增殖及胶原合成功能的影响。结果激光照射剂量由低至高可分为3个剂量段，激光强度在第一剂量段时的生物调节效应主要表现为抑制细胞增殖，在第二剂黾段时则能促进细胞增殖并抑制胶原合成，在第三剂量段时间则可促进腔原合成，且每个剂量段内的激光生物调节效应与照射剂量间大致单线性相关。上述结果均支持牛物信息模型的光生物调节作用分析。结论低强度He-Ne激光照射可以调节成纤维细胞的增殖及胶原合成功能，这可能是其促进伤口愈合的相关机制之。

The regulative effects of low intensity He-Ne laser irradiation on the function of fibroblast cells

Objective To investigate the regulative effects of low intensity He-Ne laser irradiation(LIHNLI) on the proliferation of fibroblast cells and collagen synthesis in normal human skin. Methods Cultured normal human skin fibroblast cells were treated by LIHNLI with different concentrations. The cell proliferation and collagen synthesis were measured by using of a proliferation colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and the spectrophotometric method for the determination of hydroxyproline, respectively. Results Fibroblast cells were divided into three groups, and cell proliferation was inhibited in the low-dose group, but promoted in the middle-dose group, while the collagen synthesis was inhibited in the middle-dose group, but promoted in the high-dose group. It was found that the effects of LIHNLI were lineally related to irradiation dosage in each group. The photobiomosulation effectes of the biological information model was supported by all results above. Conclusion The cell proliferation and the collagen synthesis of normal human skin fibroblast cells could be regulated by LIHNLI, which might be the relative mechanism of promoting wound healing.