Sema4D和HER-2在乳腺癌组织中的表达及临床意义

目的探讨Sema4D和人类表皮生长因子受体2（HER-2）在乳腺癌组织中的表达及临床意义。方法采用免疫组织化学sP法检测110例乳腺癌组织中Sema4D和HER-2的表达，并检测肿瘤微淋巴管密度（Microlymphtic vessel density，MLVD）。结果乳腺癌组织中Sema4D和HER-2蛋白的阳性表达率分别为71．82％（79／110）和33．64％（37／110），与对照组比较差异有统计学意义（P〈0．01）：淋巴结转移阳性组的Sema4D阳性表达率和MLVD值显著高于淋巴结转移阴性组（P〈0．01）；Sema4D和HER-2阳性表达与组织学分级、TNM分期、淋巴结转移及ER表达状态显著相关（P〈0．05），而与肿瘤大小及PR表达状态无关（P〉0．05）。相关分析结果显示Sema4D与HER-2表达呈正相关（r=0．535，P〈0．01）；单因素生存分析显示，Sema4D高表达组的5年生存率（74．19％）高于低表达组（53．16％），其差异有统计学意义（P〈0．05）。结论Sema4D可能对乳腺癌的发生、发展起重要作用。

The expressions of Sema4D and HER-2 proteins in breast cancers and their clinical significance

Objective To investigate the expressions of Sema4D and HER-2 in breast cancers and their relationship with microlymphtic vessel density （ MLVD ）. Methods MLVD and expressions of Se- ma4D and HER-2 were detected in 110 cases of breast cancer tissues by immunohistochemistry. The rela- tionship of Sema4D and HER-2 expressions with elinicopathologic parameters was analyzed. Results The positive expression rate of Sema4D and HER-2 was 71.82% （79/110） and 33.64% （37/110） , respective- ly ,with a statistically significant differences compared to those in the control group （ P 〈 0. 01 ）. The posi- tive expression rates of Sema4D and MLVD in cases with lymphatic metastasis were higher than that without lymphatic metastasis （ P 〈 0. 01 ）. The expressions of Sema4D and HER-2 were closely correlated with the histological grade, TNM stage, lymph node metastasis, and expression of ER of breast cancers （ P 〈 0. 05 ） , but were not related to tumor size and expression of PR （ P 〉 0. 05 ）. On univariate analysis, the disease-free survival rate of patients with Sema4D positive expression was better than those with its negative expression （ P 〈 0. 05 ）. Sema4D expression was positively correlated with the HER-2 expression （ r = 0. 535, P 〈 0. 01 ）. Conclusions Sema4D may play important roles in the carcinogenesis and development of a breast cancer.