“血分论治”对寻常型银屑病患者Th17／Treg影响的研究

目的采用“血分论治”方法治疗寻常型银屑病患者，观察其外周血Th17、Treg细胞变化，探讨“血分论治”银屑病的作用机制。方法选择32例寻常型银屑病患者（血热证组17例，血瘀证组15例），分别给予清热凉血方、益气活血方治疗，于治疗前后流式细胞分析法检测外周血Th17细胞和CD4＋CD25＋Treg细胞亚群的变化。同时选择15例健康人为对照组。结果治疗前两组患者Th17／Treg（4．21±0．52）％、（3．32±0．43）％]显著高于对照组（1．79±0．18）％，P〈0．01]；中药治疗后均明显下降（2．41±0．22）％、（2．02±0．12）％，P〈0．05]。患者Th17／Treg表达水平与PASI评分呈正相关（r=0．471，P〈0．05）。结论“血分论治”寻常型银屑病可通过调节Th17／Treg水平发挥治疗作用。

Experimental study on effects of blood-stage treatment on Th17/Treg in patients with psoriasis vulgaris

Objective To investigate the action mechanism of blood-stage treatment that affects psoriasis vulgaris using observation of the Th17/Treg expression. Methods A total of 32 patients （ blood heat group, n = 17; blood stasis group, n = 15）and 15 healthy people （control group, n = 15） were ob- served. The frequencies of Th17 and Treg in peripheral blood were detected by flow cytometric analysis in patients before and after treated by heat-clearing and blood-cooling decoction, qi-enriching and blood-activa- ting decoction. Results The ratio of Thl 7/Treg in peripheral blood blood heat group （4. 21 ± 0. 52） % ; blood stasis group （ 3.32±0. 43 ） % ] was siguifieantly increased in patients compared with controls （ 1.79±0. 18 ）% ] （ P 〈 0. 01 ）. After herbal treatment, the ratio of Th17/Treg expression in the blood heat group （ 2.41± 0. 22 ） % ] and in blood stasis group （ 2. 02±0. 12 ） % ] was significantly decreased （ P 〈 0. 05 ）, respectively. Conclusions Blood-stage treatment works well on Th17/Treg expression in patients with pso- riasis vulgaris.