间接ELISA检测无菌性脑膜炎患儿的埃可病毒特异性IgM抗体

目的：研究检测患儿脑脊液和血清标本的特异性IgM在诊断埃可病毒感染的无菌性脑膜炎的实用价值。方法：采用埃可病毒混合抗原包被酶标板，用间接ELISA方法检测脑脊液和血清标本的特异性IgM抗体。结果：在临床诊断为无菌性脑膜炎患儿的78份脑脊液和203份血清标本中分别有14份（17.9%）和48份（23.6%）阳性，而36份细菌性脑膜炎和30份脑外伤患者脑脊液仅1份阳性；细菌性脑膜炎、正常成人血清均为阴性。结论：本方法快速、简便、可靠，适合于临床早期诊断，而脑脊液标本IgM抗体的检测在诊断埃可病毒感染的无菌性脑膜炎可能更为准确。

Detection of the echovirus specific IgM antibodies in children with aseptic meningitis by ELISA

Objective: In order to study the practical value of detecting specific IgM in cerebrospinal fluid and serum specimens in diagnosing echovirus meningitis.Methods:48 wells plates were coated with mixed echoviruses antigen, and then specific IgM antibodies in cerebrospinal fluid and serum specimens were detected with indirect ELISA. Results: In 78 cerebrospinal fluid and 203 serum specimens with aseptic meningitis, the positive rates were 17.9% (14/78) and 23.6%(48/203),whereas only one specimen was positive in 66 cerebrospinal fluids with bacterial meningitis and brain trauma, and all control sera were negative. Conclusion: This assay is simple,sensitive,quick and reliable, which is suitable for early and specific clinical diagnosis.It may be more reliable to detect IgM in CSF of the patients for diagnosing echovirus meningitis.