| 基因枪介导不同真核质粒DNA转染体外细胞系的实验研究 |
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| 引用本文： | 蒙世芳,陈建海,刘艺杰,赖春丽. 基因枪介导不同真核质粒DNA转染体外细胞系的实验研究[J]. 细胞与分子免疫学杂志, 2010, 26(4) |
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| 作者姓名： | 蒙世芳 陈建海 刘艺杰 赖春丽 |
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| 作者单位： | 1. 南方医科大学南方医院药学部,广东,广州,510515
2. 温州医学院第二附属医院妇产科,浙江,温州,325000 |
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| 摘 要： | 目的:建立基因枪子的弹制备及将不同质粒DNA转染体外培养MCF-7细胞系的方法。方法:以亚精氨-氯化钙沉淀法制备子弹,采用基因枪方法分别将真核表达质粒pEG-FP,Pmcherry转染对照组和实验组MCF-7细胞,转染24h后,利用荧光显微镜观察细胞中红、绿荧光蛋白的表达情况。结果:制备了基因枪子弹,基因枪介导的pEGFP、Pmcherry能够分别和共同转染入体外培养的MCF-7细胞中,转染后24h可检测到红、绿色荧光,而对照组则没有荧光蛋白的表达。结论:基因枪能够有效介导外源基因转移并能够实现两种基因的共同转移,基因枪转染的MCF-7细胞能够有效表达报告基因。
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| 关 键 词： | 基因枪 细胞转染 MCF-7细胞系 |
Different gene gun-mediated plasmid DNA transfection of eukaryotic cell lines in vitro experimental study |
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| MENG Shi-fang,CHEN Jian-hai,LIU Yi-jie,LAI Chun-li. Different gene gun-mediated plasmid DNA transfection of eukaryotic cell lines in vitro experimental study[J]. Chinese journal of cellular and molecular immunology, 2010, 26(4) |
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| Authors: | MENG Shi-fang CHEN Jian-hai LIU Yi-jie LAI Chun-li |
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| Affiliation: | MENG Shi-fang,CHEN Jian-hai,LIU Yi-jie,LAI Chun-li Department of Pharmacy,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | AIM:To establish a preparation method of cartridge for gene gun and differtent gene transfectiono to MVF-7 cell lines in vitro. METHODS:The cartridge was prepared by precipitation method of spermidine and calcium chloride.using gene gun method,respectively,the eukaryotic expression plasmid pEGFP,Pmcherry transfected with the control group and experimental group MCF-7 cells 24 h after transfection. RESULTS:The preparation of the gene-gun bullets,gene gun-mediated pEGFP,Pmcherry be able to separate and co-tra... |
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| Keywords: | gene gun cell transfection MCf-7 cell lines |
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