| 姜黄素对喉癌Hep-2细胞增殖及Bcl-2、Bax基因表达的影响 |
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| 引用本文： | 张立,刘剑凯,孙吉凤,邹维. 姜黄素对喉癌Hep-2细胞增殖及Bcl-2、Bax基因表达的影响[J]. 中国实验诊断学, 2010, 14(7): 1053-1055 |
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| 作者姓名： | 张立 刘剑凯 孙吉凤 邹维 |
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| 作者单位： | 1. 长春医学高等专科学校,生物化学教研室,吉林,长春130031
2. 吉林大学白求恩医学院生物化学与分子生物学教研室,吉林,长春130021 |
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| 基金项目： | 吉林省教育厅"十一五"科学技术研究项目资助课题 |
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| 摘 要： | 目的探索姜黄素对喉癌细胞增殖的作用以及对细胞Bcl-2和Bax基因表达的影响。方法 Hep-2细胞暴露于含姜黄素(3-25μM)的培养基中,分别培养24h及48h,用MTT法检测对细胞增殖的影响;用RT-PCR法检测Bcl-2和Bax的mRNA表达水平。结果 MTT显示,姜黄素使Hep-2细胞增殖明显降低,并呈时间-剂量依赖性;RT-PCR结果显示,姜黄素使Bcl-2的mRNA表达水平降低,而使Bax的mRNA表达水平升高,并呈剂量和时间依赖性。结论姜黄素对人喉癌Hep-2细胞增殖具有显著的抑制作用。其作用机制可能与其下调Bcl-2基因表达水平及上调Bax基因表达水平,从而诱导细胞凋亡有关。
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| 关 键 词： | 姜黄素 喉癌细胞 细胞增殖 Bcl-2 Bax |
Effect on the expression of Bcl-2,Bax and on cell proliferation by curcumin in laryngeal cancer cell Hep-2 |
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| Affiliation: | ZHANG Li,LIU Jian-kai,SUN Ji-feng,et al.(Department of Biochemistry,Basic Medical School,Changchun Medicial College,Changchun 130031,China) |
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| Abstract: | Objective To study effect of curcumin on proliferation and the expression of Bcl-2,Bax in laryngeal cancer cell Hep-2.Methods Hep-2 cells were exposed to curcumin at concentrations from 3 to 25 μM,cultured for 24 h or 48 h.MTT was used to determine cell proliferation.The expression levels of Bcl-2,Bax mRNA were analyzed by RT-PCR.Results MTT showed that the cell proliferation was down regulated by curcumin significantly in a does and time dependent manner;RT-PCR showed that the expression of Bcl-2 was decreased and the Bax mRNA was increased by curcumin.Conclusion Curcumin inhibits the proliferation of laryngeal cancer Hep-2 cells significantly.The possible mechanism is related with curcumin down regulating bcl-2 but up regulating bax. |
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| Keywords: | Bcl-2 Bax |
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