PKC抑制剂灯盏花素乙对内脏炎症痛大鼠脊髓NO/cGMP信号转导系统的影响

目的探讨灯盏花素乙在甲醛复制的内脏炎症痛中的作用及其对NO/cGMP信号转导系统的影响。方法成年健康Wistar大鼠,随机分为4组:正常对照组、内脏炎症痛组、溶媒组和灯盏花素乙组。观察:①鞘内注射灯盏花素乙后大鼠行为学变化,以15min为一个时间段,共2h,计算疼痛分数;②致痛后30min取脊髓,用分光光度计法测定NOS活性、NO产量和放射免疫法测定cGMP含量。结果①灯盏花素乙组在前90min内疼痛分数低于内脏炎症痛组(P<0.05orP<0.01);②内脏炎症痛、溶媒和灯盏花素乙组脊髓的NOS活性、NO产量和cGMP含量均高于正常对照组(P<0.05orP<0.01),灯盏花素乙组低于内脏炎症痛组(P<0.01)。结论①灯盏花素乙能减轻甲醛复制的内脏炎症痛;②灯盏花素乙可能通过抑制脊髓内PKC的激活,进而抑制NO/cGMP信号转导系统的激活。

Effect of PKC inhibitor, chelerythrine chloride, on NO/cGMP signaling pathway in the spinal cord of rat with visceral inflammatory pain

Aim To explore the role of chelerythrine chloride in formalin induced visceral inflammatory pain and the effect of it on NO/cGMP signaling pathway.Methods Adult healthy Wistar rats were divided into 4 groups randomly:normal control group;formalin group;vehicle group and chelerythrine chloride group.A numerical score for pain behaviors was judged every 15 minutes for 2 hours after intrathecal injection of chelerythrine chloride.The spinal cord was quickly removed 30 minutes after formalin injection.NOS activity,NO production and cGMP content in the spinal cord were measured by spectrophotometry and radioimmunoassay.Results ① Pain scores of chelerythrine chloride group were lower than those of formalin group(P<0.05 or P<0.01)within the former 90 minutes.② NOS activity,NO production and cGMP content of the spinal cord increased in formalin,vehicle and chelerythrine chloride group as compared with those of normal control(P<0.05 or P<0.01).NOS activity,NO production and cGMP content were lower in chelerythrine chloride group than those of formalin group(P<0.01).Conclusions ① Chelerythrine chloride can alleviate the degree of visceral inflammatory pain.② Chelerythrine chloride may inhibit NO/cGMP signaling pathway in the spinal cord through inhibiting PKC.