结直肠癌中CXCR5、CXCL13、MMP-12和MMP-13的表达及意义

目的 观察结直肠癌组织中趋化因子CXCR5及其配体CXCL13以及MMP-12、MMP-13的表达，探讨其与临床病理特征、预后的关系。方法 应用免疫组织化学SP法检测236例结直肠癌及其切缘正常肠黏膜组织以及62例结直肠腺瘤组织中CXCR5、CXCL13、MMP-12和MMP-13蛋白表达。结果 （1）CXCR5、CXCL13、MMP-12、MMP-13在结直肠癌组织中的表达率为43.6%,41.5%、83.5%和80.5%均高于在切缘正常肠黏膜组织中的表达率（4.2% 、5.5%、11.9%和13.1%）及在结直肠腺瘤组织中的表达率（24.2%、17.7%、69.4%和64.5%）（P均<0.05）。（2）CXCR5、CXCL13、MMP-12、MMP-13蛋白表达与淋巴结转移、远处转移、肿瘤分期及复发呈正相关(P<0.05)。CXCL13蛋白表达与组织分化程度呈正相关(P<0.05)。（3）CXCR5与CXCL13蛋白表达呈正相关(P<0.05)。CXCR5、CXCL13蛋白表达分别与MMP-12和MMP-13呈正相关(P<0.05)。结论 CXCR5、CXCL13、MMP-12、MMP-13的表达促进结直肠癌的发生、发展以及转移、复发, 可作为预测结直肠癌转移和复发的有价值指标。

Expression of CXCR5, CXCL13, MMP-12 and MMP-13 in Colorectal Cancer and Their Significance

Objective To investigate the expression of CXCR5, CXCL13, MMP-12 and MMP-13, and to explore their relationship with clinicopathologic features and prognosis of colorectal carcinoma. Methods The immunohistochemistry staining method was used to detect the expression of CXCR5, CXCL13, MMP-12 and MMP-13 protein in 236 paired specimens of colorectal cancer and normal tissues and 62 cases of colorectal adenoma. Results The positive rates of CXCR5, CXCL13, MMP-12 and MMP-13 were 43.6%, 41.5%, 83.5% and 80.5% in colorectal carcinoma tissues, respectively, which were much higher than those in incisal edge normal intestinal mucosa tissues (4.2% , 5.5%, 11.9% and 13.1%) and those in colorectal adenomas tissues(24.2%, 17.7%, 69.4% and 64.5%) (P<0.05). The expression of CXCR5, CXCL13, MMP-12 and MMP-13 were positively correlated with lymph node metastasis, distant metastasis, TNM stage and relapse(P<0.05). Additionally, CXCL13 positive staining was correlated with poor differentiation(P<0.05). CXCR5 expression was significantly correlated with CXCL13 expression(P<0.05). The expression of CXCR5 and CXCL13 were positively correlated with MMP-12 and MMP-13, respectively (P<0.05). Conclusion The expression of CXCR5，CXCL13，MMP-12 and MMP-13 may play a crucial role in the carcinogenesis, development, metastasis and relapse of colorectal cancer. They could be used as prognostic markers of colorectal cancer in clinical practice.