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| 人C1qR单克隆抗体E8的制备及鉴定 | |
| 引用本文： | 陈政良 谢佩蓉. 人C1qR单克隆抗体E8的制备及鉴定[J]. 免疫学杂志, 1995, 11(4): 227-232 |
| 作者姓名： | 陈政良 谢佩蓉 |
| 作者单位： | 第三军医大学免疫学教研室，第一军医大学免疫学教研室 |
| 基金项目： | 全军“八五”医药卫生科研基金 |
| 摘 要： | 提取Ｒａｊｉ细胞膜蛋白，与Ｃｌｑ－Ｓｅｐｈａｒｏｓｅ ４Ｂ亲和结合制成ＣｌｑＲ－Ｃｌｑ－ｓｅｐｈａｒｏｓｅ ４Ｂ作为免疫原，脾内免疫小鼠，应用杂交瘤技术，在国内欠制备成功一株抗人ＣｌｑＲ的单克隆抗体。经Ｃｅｌｌ－ＥＬＩＳＡ，竞争ＲＢＡ及ＷＢ等方面全面鉴定，证明Ｅ８是Ｃ１ｑＲ的特异，它可识别Ｒａｊｉ、Ｕ９３７和Ｊｕｒｋａｔ细胞上约７０ＫＤ的Ｃ１ｑＲ蛋白。 |
| 关 键 词： | Clq受体 单克隆抗体 制备 鉴定 |
Production and Characterization of a Monoclonal Antibody(E_8)toHuman ClqR |
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| Chen Zhengliang,Xie Peirong,Zheng Ping. Production and Characterization of a Monoclonal Antibody(E_8)toHuman ClqR[J]. Immunological Journal, 1995, 11(4): 227-232 | |
| Authors: | Chen Zhengliang Xie Peirong Zheng Ping |
| Abstract: | A monoclonal antibody against human cell surface Clq receptor(ClqR),designated E_8, wasproduced by fusion of the mouse myeloma cell line SP2/0 with the spleen cells of Balb/c mice immunized withintrasplenic injection of ClqR-Clq-Sepharose 4B. Using Cell-ELISA,RBA, and WB,it was evidenced that E_8is specifically directed to ClqR and can recognlze the ClqR protein of 70kd on Raji, U937 and Jurkat cells. |
| Keywords: | ClqR Monoclonal antibody |
| 本文献已被 CNKI 维普 等数据库收录！ | |