首页 | 本学科首页   官方微博 | 高级检索  
检索        


Increased intestinal permeability in pathogenesis and progress of nonalcoholic steatohepatitis in rats
Authors:Jin Xi  Yu Chao-Hui  Lv Guo-Cai  Li You-Ming
Institution:1. Digestive Department of the First Affiliated Hospital, Medical School, Zhejiang University,Hangzhou 310003, Zhejiang Province, China
2. Clinical Laboratory of the First Affiliated Hospital,Medical School, Zhejiang University, Hangzhou 310003, Zhejiang Province, China
Abstract:AIM: To investigate whether increased intestinal permeability contributes to the pathogenesis and progress of nonalcoholic steatohepatitis by observing its dynamic change in rat models. METHODS: Rat models of nonalcoholic steatohepatitis were established by giving a fat-rich diet. The rats were sacrificed at wk 8, 12 and 16 during the study. Rats fed with normal diet were taken as control. Plasma D-lactate, plasma diamine oxidase, serum lipids and liver transaminases were measured in blood of the femoral artery. Hepatic steatosis and inflammation were assessed by haematoxylin-eosin staining. RESULTS: A rat model of nonalcoholic steatohepatitis was established successfully. Plasma D-lactate level in model group at wk 8, 12 and 16 and diamine oxidase level in model group at wk 12, 16 increased significantly compared with those in control group. There were notable differences of D-lactate and diamine oxidase level in model group between wk 8 and 12 as well as between wk 12 and 16. Serum lipids, liver transaminases and liver injury also increased with disease development. CONCLUSION: Increased intestinal permeability caused by intestinal bacterial overgrowth and endotoxin-induced intestinal destruction exists in rats with nonalcoholic steatohepatitis, which may partially explain the pathogenesis and progress of this disease.
Keywords:Nonalcoholic steatohepatitis  Intestinal permeability  D-lactate  Diamine oxidase
本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号