培养温度对脊髓灰质炎病毒繁殖及D抗原的影响

目的 选择脊髓灰质炎病毒繁殖的最佳温度，同时观察温度对病毒繁殖及D抗原的影响。方法   分别在25℃、33℃和37℃培养脊髓灰质炎病毒(Sabin株Ⅰ、Ⅱ和Ⅲ型)，从24 h开始间隔12 h取样，至细胞完全病变或96 h，用ELISA检测病毒D抗原含量，绘制变化曲线；用微量细胞病变法检测感染性滴度，绘制病毒生长曲线。结果   三个型的脊髓灰质炎病毒在25℃均不繁殖；33℃和37℃均适合病毒繁殖。对于Ⅰ和Ⅲ型病毒，33℃的繁殖滴度及D抗原水平比37℃高；Ⅱ型病毒在两种温度下繁殖特性基本一致。结论   温度对脊髓灰质炎病毒繁殖起决定性作用，综合Ⅰ、Ⅱ和Ⅲ型病毒的温度繁殖特点，33℃培养比37℃更适合脊髓灰质炎病毒繁殖。

Influence of temperature on poliovirus propagation and D-antigen production

Objective To determine the optimal temperature for poliovirus propagation and D-anti-gen production. Methods Poliovirus(Sabin strain)type Ⅰ、Ⅱ and Ⅲ were cultivated on Veto cells at 25℃ ,33℃ ,and 37℃,respectively,and sampled at an interval of 12 h until the cells aggregated com-pletely or 96 h after inoculation of the cells. D-antigen was assessed with ELISA,poliovirus were titrated with the micro-cell plaque assays. Both diagrams were presented for either cultivation or D-antigen pro-duction analysis. Results No virus propagation were detected in the cell supernatant at 25 ℃ for all the three types of poliovirus,however,virus propagation were prominent at both 33℃ and 37 ℃,while 33 ℃ being optimal for type Ⅰ and Ⅲ in terms of D-antigen production and poliovirus propagation. Type Ⅱ showed no difference between 33 ℃ and 37 ℃ on propagation. Conclusions Temperature is very im-portant for poliovirus propagation and D-antigen production and 33 ℃ is superior to 37 ℃ for poliovirus propagation.