| EB病毒转化的永生性人B淋巴母细胞中的MTHFR基因的表达 |
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| 引用本文： | 朱慧萍,李燕.EB病毒转化的永生性人B淋巴母细胞中的MTHFR基因的表达[J].卫生研究,2000,29(4):196-198. |
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| 作者姓名： | 朱慧萍 李燕 |
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| 作者单位： | [1]北京大学生育健康研究所 [2]中国预防医学科学院病毒研究所 |
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| 摘 要： | 为检测用ＥＢ病毒转化建立的永生性人Ｂ淋巴细胞株中Ｎ＾５，Ｎ＾１０－亚甲基甲氢叶酸还原酶（ＭＴＨＦＲ）基因的表达及其ｃＤＮＡ序列，用ＥＢ病毒转化人外周血Ｂ淋巴细胞，建立永生性细胞株后，从培养细胞中提取总ＲＮＡ，用ＲＴ－ＰＣＲ法扩增ＭＴＨＦＲ基因ｃＤＮＡ的不同片段，进行ＰＡＧＥ电泳分析和ｃＤＮＡ序列测定。结果显示永生性人Ｂ淋巴母细胞中有ＭＴＨＦＲ基因表达，其ｃＤＮＡ序列与文献报道的人肝脏ＭＴＡＨＦＲ基
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| 关 键 词： | EB病毒转化 B淋巴母细胞 MTHFR 基因表达 |
MTHFR gene expression in EB virus transformed human B lymphoblasts] |
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| H Zhu,R Zhao,Y Li,Z Li.MTHFR gene expression in EB virus transformed human B lymphoblasts][J].Journal of Hygiene Research,2000,29(4):196-198. |
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| Authors: | H Zhu R Zhao Y Li Z Li |
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| Institution: | Institute of Reproductive Health, Peking University, Beijing 100083, China. |
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| Abstract: | In order to detect the expression of N5, N10-methylene-tetra hydrofolate reductase(MTHFR) gene in Epstein-Barr virus(EBV) transformed human B lymphoblast, as well as the cDNA sequence, human peripheral blood was transformed by EBV to build B lymphoblast cell line. Total RNA was isolated from the cell culture. Variant segments of MTHFR cDNA were amplified using RT-PCR protocol and analysed by polyacrylamide gel electrophoresis(PAGE). One of the cDNA segments was sequenced. Results showed MTHFR gene was expressed in human B lymphoblast cell line. The cDNA sequence was almost the same as that of human liver cDNA from Genbank. |
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