雷公藤甲素对哮喘小鼠白介素8及白介素10表达的影响

目的研究雷公藤甲素对哮喘小鼠的治疗作用及对白介素8（IL-8）及白介素10（IL-10）表达的影响。方法 60只小鼠,随机分6组,每组各10只。分别为正常对照组（A）、哮喘组（B）、地塞米松治疗组（C）及不同剂量雷公藤甲素治疗组（D、E、F）,建立小鼠卵蛋白哮喘模型,用ELISA测定肺组织匀浆及气管肺泡灌洗液（BALF）中IL-8、IL-10表达水平。结果哮喘组小鼠肺组织匀浆及BALF中IL-8表达均高于正常对照组（P〈0.01）,哮喘组小鼠肺组织匀浆及BALF中IL-10表达均低于正常对照组（P〈0.01）,雷公藤甲素治疗组及地塞米松组IL-8低于哮喘组（P〈0.01）,雷公藤甲素治疗组及地塞米松组IL-10高于哮喘组（P〈0.01）。结论雷公藤甲素对哮喘小鼠有明显治疗作用,可抑制IL-8表达及提高IL-10的表达。

Effects of triptolide on expression of interleukin 8 and interleukin 10 of asthmatic mouse

Objective To investigate the curative effect and exploring effects of triptolide on the expression of IL- 8 and IL-10 onvalbumin challenged asthmatic mice. Methods 60 mice were randomly divided into 6 groups： control group （group A）, asthmatic group （group B）, dexamethasone group （group C） and triptolide group （group DEF）. The asthmatic model was established with the treatment of ovalbumin. ELISA was used to measure the expression of IL-8 and II.q0 of lung tissue and BALF respectively. Results The levels of IL-8 in asthmatic group was respectively increased compared with those in control group, and the levels of IL-10 in asthmatic group was respectively reduced compared with those in control group （ P 〈0.01）. The levels of IL-8 in dexamethasonend group and triptol- ide group was significantly reduced compared with those in asthmatic group, The levels of II.-10 in dexamethasonend group and triptolide group was significantly increased compared with those in asthmatic group （ P 〈0.01 ） . Conclusion Triptolide has significant curative effect ,and triptolide can inhibit the expression of IL-8 and promote the expression of IL-10.