肺炎患儿肺炎支原体感染的实验室检测

目的：探讨肺炎患儿肺炎支原体（ＭＰ）感染的实验室检测方法。方法：使用聚合酶链反应（ＰＣＲ）技术检测６７６例肺炎患儿ＭＰ的感染，其中１３９例同时采用支原体培养进行检测；１１２例做间接血凝试验（ＩＨＡ）。结果：ＰＣＲ法对ＭＰ感染的检出率明显高于培养法（Ｐ〈０．０１），双份血清ＩＨＡ的检测率和ＰＣＲ法比较无显著性差异（Ｐ〉０．０５），单份血清ＩＨＡ的检出率明显低于ＰＣＲ法（Ｐ〈０．０１）。结论：ＰＣＲ法

Detection of Mycoplasma Pneumoniae in Children with Pneumonia

Objective:To evaluate the methods for detection and diagnosis of mycoplasma infections in children with pneumonia.Methods:The polymerase chain reaction (PCR) technique,mycoplasma culture and indirect haemagglutination assay (IHA) were used to detect mycoplasma pneumoniae in clinical samples obtained from 676 children (411 male and 265 female) with pneumonia.Results:Of 676 patients with pneumoniae,115 children were confirmed to be positive of mycoplasma pneumoniae.The positive rate of detection was significantly higher with PCR than with mycoplasma culture ( P < 0.01 ).There was no significant difference between the positive rate of detection with PCR and that with IHA ( P >0.05).Conclusion:PCR method and IHA are highly applicable in routine clinical laboratories for the preliminary detection and diagnosis of mycoplasmal infections,particularly in extrapulmonary cases.