绞股蓝总皂甙对小鼠产生IFN—r的影响

本文报道了绞股蓝总皂甙对正常和大黄脾虚模型小鼠IFN—r产生的影响。实验结果表明:ConA能诱导小鼠脾细胞产生IFN—γ;大黄脾虚模型小鼠IFN—γ水平比正常小鼠明显降低.绞股蓝总皂甙10～40mg/L以7/50～200mg/kg在体外、体内均可明显提高ConA诱导正常小鼠脾细胞产生的IFN—γ水平.其增加ConA诱导IFN—γ产生的最适浓度在体外为20mg/L,在体内为100mg/kg;绞股蓝总皂甙10～80mg/L以及50～100mg/kg在ConA协同下在体外、体内均能使大黄脾虚模型小鼠低下的IFN—γ水平明显提高,并使之恢复、甚至超过正常水平,其提高ConA诱导IFN一γ产生的最适浓度在体外、体内分别是20mg/L和50～100mg/kg.绞股蓝总皂甙能使大黄脾虚小鼠低下的IFN—γ水平恢复的实验结果进一步提示IFN—γ可能与机体的“正气”和“脾”的本质存在某种内在联系,IFN—γ可能是中医“脾气”的免疫学物质基础之一。

Effect of Gypenoside on the Production of Interferon-r in Mice

An experiment was made to study the effect of Gypenoside on interf eron (IFN-r) production of normal mice and Rhubarb-inducing spleen-asthenia model mice in order to understand the relationship between Gypenoside and IFN-r. The results showed that: 1) Gypenoside (10-40 ug/ml in vitro, 50-200mg/kg in vivo) could increase IFN-r production induced by ConA from normal murine spleen cells; 2) IFN-r production in spleen-asthenia mice was lower than that in normal mice which could be enhanced to normal level by ConA and Gypenoside (10-80ug/ml in vitro, 50-100mg/kg in vivo); 3) No IFN could be induced by Gypenoside alone. It is suggested that IFN-r may be related to "healthy qi" and "spleen", and also one of the immunological substantial basis of "spleen-qi" in traditional Chinese medicine.