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Acute effects of mitoxantrone on the template activity of isolated nuclei from the T-47D human breast tumor cell line |
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| Authors: | N Chegini A R Safa M T Tseng |
| Affiliation: | 1. Department of Obstetrics and Gynecology, University of Louisville Health Sciences Center, Louisville, KY 40292 U.S.A.;2. Department of Anatomy, University of Louisville Health Sciences Center, Louisville, KY 40292 U.S.A.;3. J. Graham Brown Regional Cancer Center, University of Louisville Health Sciences Center, Louisville, KY 40292 U.S.A. |
| Abstract: | The effect of mitoxantrone on the template activity of nuclei isolated from the T-47D human breast tumor cell line was investigated. The results suggest that mitoxantrone significantly inhibits total RNA synthesis of these nuclei in a concentration-dependent manner. At low drug concentrations (10(-9) M and 10(-7) M) RNA synthesis was inhibited by 21.9% and 41% compared to control values, respectively. Greater inhibition was observed when the mitoxantrone concentration was increased to 10(-5) M or 10(-4) M (56% and 77%, respectively). Experiments utilizing alpha-amanitin revealed that mitoxantrone inhibits RNA polymerase II activity in a concentration-dependent manner. |
| Keywords: | Address all correspondence and reprint requests to: Dr. Michael T. Tseng Department of Anatomy University of Louisville Health Sciences Center Louisville KY 40292 U.S.A. |
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