| 半边旗提取物5F对人高转移卵巢癌细胞HO-8910PMETS-1mRNA和VEGF蛋白表达的影响 |
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| 引用本文： | 何太平,莫丽儿,梁念慈. 半边旗提取物5F对人高转移卵巢癌细胞HO-8910PMETS-1mRNA和VEGF蛋白表达的影响[J]. 中药材, 2005, 28(4): 307-309 |
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| 作者姓名： | 何太平 莫丽儿 梁念慈 |
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| 作者单位： | 1. 广东医学院生物化学与分子生物学研究所,广东湛江,524023
2. 广东天然药物研究与开发重点实验室,广东湛江,524023 |
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| 基金项目： | 中国科学院资助项目，广东省湛江市988科技兴湛计划 |
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| 摘 要： | 目的:研究半边旗提取物5F(以下简称5F)对HO-8910PM细胞内VEGF蛋白及ETS-1mRNA表达的影响,探讨其抗肿瘤侵袭转移的作用机制.方法:RT-PCR分析ETS-1mRNA的表达;Western blot分析VEGF蛋白的表达.结果:25～100 μmol/L 5F作用HO-8910PM细胞24 h后,明显下调ETS-1mRNA的表达;5F明显下调VEGF蛋白表达水平.结论:5F抗肿瘤侵袭转移能力与ETS-1mRNA和VEGF蛋白的表达有关.
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| 关 键 词： | 半边旗提取物5F 基因表达 肿瘤药物治疗 卵巢癌 |
| 修稿时间： | 2004-11-05 |
Effects of 5F from Pteri semipinnata on the Expression of ETS-1 mRNA and VEGF Protein of Highly Metastatic Ovarian Carcinoma HO-8910PM Cells |
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| He Taiping,Mo Lier,Liang Nianci. Effects of 5F from Pteri semipinnata on the Expression of ETS-1 mRNA and VEGF Protein of Highly Metastatic Ovarian Carcinoma HO-8910PM Cells[J]. Journal of Chinese medicinal materials, 2005, 28(4): 307-309 |
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| Authors: | He Taiping Mo Lier Liang Nianci |
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| Affiliation: | Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023. |
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| Abstract: | OBJECTIVE: To study the effect of 5F from Pteri semipinnata L. on the expression of ETS-1 mRNA and VEGF protein, and to investigate the antitumor mechanisms of 5F from Pteri semipinnata L. METHODS: RT-PCR assay was used to assess the expression level of ETS-1 mRNA. The expression level of VEGF protein was assessed by Western blot analysis. RESULTS: The expression level of of VEGF protein obviously decreased in HO-8910PM cells treated with 25 to approximately 100 micromol/L 5F for 24 hours. 5F significantly down-regulated the expression of ETS-1 mRNA. CONCLUSION: The antitumor activity of 5F from Pteri semipinnata L is involved in the expression of ETS-1 mRNA and VEGF protein. |
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| Keywords: | F from Pteri semipinnata L Gene expression Tumor/pharmacotherapy Ovarian carcinoma |
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