The REV2 gene of Saccharomyces cerevisiae: cloning and DNA sequence |
| |
Authors: | F Ahne M Baur F Eckardt-Schupp |
| |
Institution: | (1) GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Strahlenbiologie, Ingolstädter Landstrasse 1, W-8042 Neuherberg, Federal Republic of Germany |
| |
Abstract: | Summary The REV2 gene of Saccharomyces cerevisiae was cloned and sequenced; it contains an open reading frame of 1985 bp with a coding potential of 662 amino acids. Interruption of the chromosomal REV2 gene by integrating the URA3 gene coupled with partial deletion of the 3 terminal region produced viable haploid rev2 mutants. This indicates that the REV2 gene is non-essential for growth. The rev2 mutant is slightly more UV-sensitive than strains carrying various rev2 alleles (rev2-1, rev2x, rad5-1, rad5-8). The putative Rev2 protein is probably a globular protein containing a highly conserved nucleotide-binding site and two zinc-finger domains. |
| |
Keywords: | Yeast DNA-repair Mutation-deficient mutant Nucleotide-binding consensus |
本文献已被 SpringerLink 等数据库收录! |