生长抑素类似物对人肝癌细胞株MHCC97-H的影响

目的:探讨生长抑素类似物奥曲肽对人高转移潜能肝癌细胞株MHCC97-H的影响及可能机制.方法:MHCC97-H肿瘤组织种植于裸鼠肝脏建立肝癌动物模型27只,随机分为3组:阴性对照组和小、大剂量干预组,同等条件下饲养35d,记录动物模型生存期,死亡时质量;观察肝脏,肺脏病理改变;免疫组化方法检测肝脏肿瘤组织基质金属蛋白酶-2(MMP-2)表达变化.结果:奥曲肽干预组动物模型较阴性对照组动物模型死亡时平均体重增加,肝癌转移率下降(P＜0.05);干预组间对比未见统计学差异(P＞0.05);免疫组化显示干预组肝脏肿瘤细胞MMP-2表达较阴性对照组下调(P＜0.05);干预组组间比较未见统计学差异(P＞0.05).结论:奥曲肽可改善荷MHCC97-H细胞裸鼠肝癌模型生存质量,抑制MHCC97-H细胞肺转移,下调肿瘤组织MMP-2的表达是奥曲肽抑制MHCC97-H细胞侵袭转移的可能机制之一.

Influence of somatostatin analogue on human hepatocellular cancer cell line MHCC97-H

AIM: To study the influence of somatostatin analogue octreotide on human hepatocellular cancer cell line MHCC97 -H and its mechanisms in vivo. METHODS: Twenty-seven nude mice,of which MHCC97-H tumor mass was planted in liver, were divided into 3 groups randomly: negative control group, low dose interfered group, large dose interfered group,and raised at similar condition for 35 d the survival time , the weight at the death point were racorded and the pathologicail changes of livers and lungs were changed; the expression of matrix metalloproteinases-2(MMP-2)in hepatocellular carcinoma was detected by immunohistochemical technique. RESULTS: Compared with the animal models of negative control group, the animal models of interfered groups were heavier in mean weight at death point and lower in metastasis rate (P<0.05); while there was no statistical difference between the 2 interfered groups (P>0.05); the expression of MMP-2 in hepatocellular carcinoma of interfered groups was gradually increased than that in control group(P< 0.05); while there was no statistical difference between interfered groups(P>0.05). CONCLUSION: Octreotide can improve the living quality of animal models and inhibit the metastasis of human hepatocellular cancer cell line MHCC97-H in vivo,which might be related to the down-regulation of the expression of MMP-2.