角膜基质细胞—PGA生物支架复合体外培养研究

目的 研究角膜基质细胞与PGA亲和力，为组织工程技术建构角膜提供重要的理论依据和参数。方法 体外分离获得角膜基质细胞，经培养扩增后接种于PGA，并对细胞－生物材料复合物进行体外培养，观察细胞生长代谢。应用MTT技术测定角膜基质细胞与PGA的黏附率。结果 角膜基质细胞能够在PGA中黏附、伸展和分泌基质，MTT测得细胞黏附率为71．40％。结论 角膜基质细胞与PGA具有较好的亲和力，PGA可以作为构建角膜的生物材料。

Experimental study on transplantation on corneal stromal cells onto PGA

Objective To study the affinity of corneal stromal cells and PGA (polyglycolic acid), in order to fabricate a model of cells culture in a three dimensional environment. Methods Approximately 1 million cultured corneal stromal cells were seeded onto the three-dimensional PGA fiber scaffolds. At the 4th day after cell seeding, MTT automated colormetric microassay was adopted to count the number of the cells attached to the PGA fibers. And at the 6th and 18th days, the complexes were observed by a scan electron microscopy(SEM). Result Corneal stromal cells secreted the extracellular matrix(ECM) and multiplied shortly after they were adhered to the PGA. The cell adhersion rate was 71.40%. Conclusion PGA can be fabricated into a suitable three-dimensional scaffold for corneal stromal cells to live in. This cell-polymer complex can be used for tissue engineered cornea research.