三种噬菌体对二氯异氰尿酸钠消毒剂抵抗力的比较

目的筛选出消毒实验中评价消毒因子对病毒灭活效果的指示噬菌体,研究噬菌T4、ψx174D和f2对二氯异氰尿酸钠（NaDCC）消毒剂的抵抗力。方法消毒剂对噬菌体的灭活效果采用悬液定量灭活实验、中和剂鉴定实验,噬菌体的检测采用双层琼脂法。结果（1）有效氯浓度为150mg／L的NaDCC溶液与噬菌体舛作用40min,或有效氯浓度为300mg／L作用5min,即可达到消毒水平对噬菌体舛的灭活对数值或噬菌体舛的对数减少值（log10 N0-log10 Nt）≥4．00log10]。（2）有效氯浓度为300mg／L的NaDCC溶液与噬菌体ψx174D作用5min,或有效氯浓度为400mg／L作用3min达到消毒。（3）有效氯浓度为2000mg／L的NaDCC溶液与噬菌体口作用20min,或有效氯浓度为4000mg／L作用5min达到消毒。结论上述3种噬菌体对NaDCC的抵抗力为：噬菌体口〉噬菌体T4〉噬菌体ψx174D。

Comparison on resistance of bacteriophages to sodium dichloroisocyanurate in laboratory

OBJECTIVE: To scan the most resistable bacteriophage as an indictor in disinfection tests, and to study the resistance of bacteriophage T4, Phichi 174D, and f2 to the sodium dichloroisocyanurate (NaDCC) in laboratory. METHODS: The virucidal activity of NaDCC against bacteriophage T4, Phichi 174D, and f2 were assessed by suspension test. The neutralizer was selected and be appraised by test of neutralizer. Bacteriophage T4, Phichi 174D, and f2 were detected and enumerated by the double-agar-layer plaque technique. RESULTS: (1) With 150 mg/L of available chlorine of NaDCC solution, within a contact time of 40 minutes, or 300 mg/L, 5 minutes, the reductions of bacteriophage T4 achieved the "disinfection" level log(10) inactivation value or log(10) reduction value of bacteriophage T4 (log(10)No-log(10)Nt) > or = 4.00 log(10)]. (2) With 300 mg/L of available chlorine of NaDCC solution, within a contact time of 5 minutes, or 400 mg/L, 3 minutes, the reductions of bacteriophage Phichi 174D achieved the "disinfection" level. (3) With 2000 mg/L of available chlorine of NaDCC solution, within a contact time of 20 minutes, or 4000 mg/L, 5 minutes, the reductions of bacteriophage f2 might achieve the "disinfection" level. CONCLUSION: The order of resistance of the above three bacteriophages to NaDCC from greatest to smallest is as follows: bacteriophage f2 > bacteriophage T4 > bacteriophage Phichi 174D.