高三尖杉酯碱通过激活Caspase-3诱导T-淋巴细胞白血病细胞Molt-3凋亡

目的：研究高三尖杉酯碱（ＨＨＴ）诱导Ｔ－淋巴细胞白血病细胞凋亡的作用机制。方法：采用流式细胞仪等技术对ＨＨＴ诱导Ｔ－淋巴细胞白血病细胞（Ｍｏｌｔ－３）的凋亡作用进行探讨。结果：ＨＨＴ诱导Ｍｏｌｔ－３凋亡，凋亡率与作用浓度和时间成正比；细胞被阻滞在Ｇ１期。ＨＨＴ作用２４ｈ后细胞内Ｃａｓｐａｓｅ－３活性增高，Ｃａｓｐａｓｅ－３抑制剂Ｚ－ＤＥＶＤ－ＦＭＫ能特异地阻断ＨＨＴ对Ｍｏｌｔ－３凋亡的诱导；Ｆａｓ

Homoharringtonine induced apoptosis in leukemic T-cells Molt-3 via activation of Caspase-3

Objective:To explore the mechanism of HHT induced apoptosis in leukemic T cells Molt 3 Methods:Flow cytometry ,colorimetric assay and RT PCR were used to analyse the HHT induced apoptotic pathways Results:We studied dose and time dependent effects of HHT on cell viability, cell cycle distribution, Caspase 3 activity as well as expression of Fas on Molt 3 cells In these cells, HHT induced apoptosis was associated with G1 delay and correlated with the activation of Caspase 3 Treatment with Caspase 3 inhibitor prevent HHT induced apoptosis significantly Pretreatment of Molt 3 cells with HHT did not increase the susceptibility to apoptosis induced by crosslinking of Fas receptors Furthermore, Fas L neutralizing antibodies (Nok 1 and 2) did not affect HHT induced apoptosis in these cells Conclusion:HHT induced apoptosis in Molt 3 cells involves the activation of Caspase 3