自发性脑出血患者血清MMP-9及TIMP-1水平的研究

目的研究自发性脑出血患者血清基质金属蛋白酶-9(MMP-9)及其抑制因子基质金属蛋白酶抑制因子-1(TIMP-1)的含量及临床意义。方法采用酶联免疫吸附法对62例48小时内入院的脑出血患者血清MMP-9和TIMP-1水平进行测定,与正常对照组进行比较,同时用免疫比浊法测高敏C-反应蛋白(hs-CRP)水平,并与CT所示血肿周围水肿体积和神经功能评分进行相关分析。结果脑出血患者血清MMP-9及TIMP-1水平较正常对照组明显升高(P<0.01),脑出血组MMP-9的含量与CT所示出血灶周围水肿区体积呈显著正相关(r=0.627,P<0.01)。结论脑出血患者血清MMP-9及TIMP-1水平明显增高,血清MMP-9可能成为早期反映脑出血患者血肿周围水肿严重程度和预后的指标。

Study on Matrix Metalloproteinase-9(MMP-9) and Tissue Inhibitor of Matrixmetalloproteinase-1(TIMP-1) in Patients with Spontaneous Intracerebral Hemorrhage

Objective To observe serum MMP-9 and TIMP-1 levels in patients with spontaneous intracerebral hemorrhage(ICH) and explore its clinical significance.Methods Serum MMP-9 and TIMP-1 were determined by enzyme-linked immunosorbent assay(ELISA) in 62 ICH patients hospitalized within 48h and 68 healthy people(control).C-reactive protein was determined by immunoturbidimetry.The relationship between serum MMP-9 and the perihematomal edema(PHE) volume measured by cranial CT scan and neurological assessment was investigated.Results Serum levels of MMP-9 and TIMP-1 were higher in ICH patients than those in healthy people(P<0.01).Serum MMP-9 level was positively correlated with perihematomal edema volume(r=0.627,P<0.01).Conclusions The levels of MMP-9 and TIMP-1 increased significantly in patients with ICH,it could be an earlier important factor to judge the severity of PHE and evaluate the prognosis.