免疫重建SCID小鼠B淋巴母细胞瘤模型的建立

目的在免疫重建的严重联合免疫缺陷(severecombinedimmunodeficient,SCID)小鼠体内建立人的B淋巴母细胞瘤模型。方法将SCID小鼠随机分为4组,每组7只,分别为:(1)实验组:在每只SCID小鼠的腹腔内接种人外周血单个核细胞(peripheral-bloodmononuclearcells,PBMC)1周后,腹腔接种EB病毒(Epstein-Barrvirus,EBV)悬液;(2)不经任何处理的SCID小鼠对照组;(3)腹腔接种人PBMC的SCID小鼠对照组;(4)腹腔接种EBV悬液的SCID小鼠对照组。每日观察小鼠的健康情况,于免疫重建的第5周解剖各组小鼠,观察腹腔各脏器的肿瘤形成情况,并用HE染色、组织化学以及PCR方法鉴定所长肿瘤的特性。结果4周后,在接种EBV的免疫重建SCID小鼠的腹腔中可形成(35±12.5)mm3大小的肿瘤,多位于肝脏,而对照组均未见有肿瘤生长;HE染色表明,所长的肿瘤均为高分化的B淋巴母细胞瘤;组织化学实验表明,肿瘤细胞均可表达CD19、CD20、CD45RA分子和EB病毒编码的蛋白;PCR检测显示,肿瘤组织的DNA在269bp处出现特异的EBV扩增条带。结论成功建立了免疫重建SCID小鼠的人B淋巴母细胞瘤模型,为研究人类肿瘤免疫提供了一个理想的动物模型。

Model of B immunoblastic lymphomas in the Hu-PBL-SCID mice

OBJECTIVE: To constitute a model of B immunoblastic lymphomas in the Hu-PBL-SCID mice. METHODS: The SCID mice were reconstituted by intraperitoneal injection (i.p.) of 5 x 10(7) human lymphocytes from Epstein-Barr virus (EBV) seronegative individuals. After one week, the SCID mice were inoculated with EBV by i.p. injection, and subjected to the investigation of whether there was any tumor in the abdomen of such SCID mice four weeks later. The characteristics of the found tumor was observed by the methods of Hematoxylin-eosin (HE) stain, immunohistochemical staining and polymerase chain reaction (PCR). RESULTS: Compared with the control groups, all the EBV-infected Hu-PBL-SCID mice had abdominal solid tumors (32 +/- 12.5) mm3] developed, often located in the liver. HE staining and immunohistochemical staining showed the tumors were human B cell lymphomas. EBV DNA could be detected in the tumors by the PCR. CONCLUSIONS: The model of B immunoblastic lymphomas in the Hu-PBL-SCID mice is successfully constituted, and may well be useful to the human tumor immunological study.