DNA relatedness,karyotyping and gene probing of Candida tropicalis,Candida albicans and its synonyms Candida stellatoidea and Candida Claussenii |
| |
| Authors: | M. Mahrous A. D. Sawant W. R. Pruitt T. Lott S. A. Meyer D. G. Ahearn |
| |
| Affiliation: | (1) Laboratory for Microbial and Biochemical Sciences, Georgia State University, 30303 Atlanta, GA, USA;(2) Division of Bacterial and Mycotic Diseases, Centers for Disease Control, 30333 Atlanta, Ga, USA |
| |
| Abstract: | Isolates of Candida albicans with varied phenotypes, including sucrose-negative variants (C. stellatoidea, serotypes A and B) and avirulent germ tube-negative forms (C. claussenii) showed significant (> 90%) DNA relatedness to classical C. albicans, but insignificant relatedness to C. tropicalis and sucrose-negative C. tropicalis. A transverse alternating-field gel electrophoresis procedure (TAFE) showed discrete karyotype patterns among the phenotypic variants of C. albicans including the sucrose-negative C. stellatoidea. The number of chromosome-sized DNA bands for C. tropicalis (7 bands) were within the range of bands observed for C. albicans (5 to 10 bands). The general DNA-migration pattern for C. albicans appeared distinct from that of C. tropicalis. An aspartyl proteinase (PrA) gene probe from C. albicans hybridized with chromosomal DNA from C. albicans, C. claussenii and C. stellatoidea but not with that from C. tropicalis. |
| |
| Keywords: | Karyotyping Candida albicans Candida tropicalis Candida stellatoidea Candida claussenii |
| 本文献已被 SpringerLink 等数据库收录！ |
|
