Assessment of the anti-tumor potential of glutathione |
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Authors: | J R Cook D P Huang A L Burkhardt M E Goldman M Carroll E Bresnick J F Chiu |
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Institution: | Department of Biochemistry, University of Vermont College of Medicine, Burlington, VT 05405 U.S.A. |
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Abstract: | This study assesses the effect of reduced glutathione (GSH) on regenerating liver, 3'-methyl-4-dimethylaminoazobenzene (3'-MDAB) hepatocarcinogenesis, and normal and transformed hepatocytes in vitro. GSH administered intragastrically caused only a 30% reduction in thymidine incorporation into liver DNA at 24 h after partial hepatectomy; there was no apparent effect on RNA and protein synthesis. Furthermore, in 3'-MDAB induced hepatocarcinogenesis, all GSH-treated animals developed hepatocyte nodules, and serum alpha-fetoprotein (AFP) levels were not reduced. In vitro, GSH was shown to be cytotoxic to both normal and transformed hepatocytes at serum concentrations under 10%. GSH inhibited 3H]thymidine incorporation slightly in 2 transformed hepatocyte lines, but not in normal hepatocytes. |
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Keywords: | To whom requests for reprints should be addressed |
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