ATP-MgCl2对大鼠缺血再灌注肾脏损伤保护作用的研究

目的探讨三磷酸腺苷-氯化镁(ATP-MgCl2)对缺血再灌注(ischemic-reperfusion,I/R)损伤大鼠肾脏是否具有保护作用及对肾组织血管内皮生长因子-A(vascular endothelial growth factor-A,VEGF-A)和中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinase-asso-ciated lipocalin,NGAL)mRNA表达的影响。方法将42只健康雄性Wistar大鼠随机分为3组:假手术组(S组)、模型组(M组)和ATP-MgCl2组(A组)。检测各组大鼠缺血再灌注后2、6、12h血清肌酐(Scr)和尿素氮(Bun)的水平,肾组织病理学变化以及肾组织VEGF-A和NGAL mRNA的表达。结果M组和A组大鼠肾脏缺血再灌注后各时间段的Scr、Bun水平均高于S组(P<0·05和P<0·01)。A组再灌注2、6、12h后的Scr、Bun水平明显低于M组(P<0·05)。与M组相比,A组再灌注后肾脏组织的NGAL mRNA表达下降,VEGF-A mRNA表达增高(P<0·05)。A组与M组相比肾脏组织的病理改变有明显改善(P<0·01)。结论ATP-MgCl2对大鼠肾脏缺血再灌注损伤具有保护作用,并能影响VEGF-A和NGAL mRNA的表达,其机制可能与提高机体内ATP水平有关。

Protective Effect of ATP-MgCl2 on Renal Ischemia-Peperfusion Injury in Rats

Objective To evaluate the protective effect of ATP-MgCl2 on acute ischemia-reperfusion injury and its influence on the expression of the levels of messenger RNA for neutrophil gelatinase-associated lipocalin (NGAL) and vascular endothelial growth factor-A (VEGF-A). Method Rat model ischemia/reperfusion kidney injury was established. The rats were allocated into three groups at random: sham group (group S), ischemia/reperfusion group (group M) and ATP-MgCl2 group (group A).Results It was found that 2, 6 and 12 h after the perfusion, Scr and BUN levels were higher in group M and group A than in group S(P<0.05 or P<0.01). Compared to group M, at the time points of 2 h, 6 h and 12 h, the levels of Scr and BUN were significantly higher in group A(P<0.05). ATP-MgCl2 could significantly increase the expression of VEGF-A mRNA, and down-regulate the expression of NGAL mRNA in the reperfused kidney in group A than in group M(P<0.05). The histopathological outcome of reperfused kidney in group A was much better than that of group M(P<0.01).Conclusion ATP-MgCl2 could improve the function of the reperfused kidney, and influence the expression of messenger RNA of NGAL and VEGF-A. The mechanism might lie in its ability to increase the levels of ATP in the injured kidney.