重症肌无力患者血清Ryanodine受体抗体检测及意义

目的探讨Ryanodine受体（RyR）抗体在重症肌无力（myasthenia gravis，MG）诊断中的临床意义。方法采用ELISA法检测89例MG患者、66例其他神经系统疾病患者和66名正常对照者血清RyR抗体水平。结果MG组血清RyR抗体阳性率显著高于其他神经系统疾病组和正常对照组（P〈0．05），其敏感性和特异性分别为55．0％和91．7％。晚发型MG患者血清RyR抗体阳性率（74．4％）明显高于早发型MG（37．0％，P〈0．05）。MG合并胸腺瘤（MGT）和未合并胸腺瘤（nMGT）患者血清RyR抗体阳性率差异无统计学意义（P〉0．05）。将MG患者根据Osserman评分进行分型，各型血清RyR抗体阳性率及其吸光度值大小与病情严重程度无相关性（P〉0．05）。结论RyR抗体多见于晚发型MG，对诊断MG具有较高的敏感性和特异性。

Detection of Ryanodine Receptor Antibody in Sera from Myasthenia Gravis Patients and Its Clinical Significance

Objective To evaluate the significance of the Ryanodine receptor(RyR) antibody in the sera of patients with myasthenia gravis(MG).Methods We detected RyR antibody by ELISA in the sera from 89 cases of MG group,66 cases of other neurological diseases and 66 cases of normal control groups.Results RyR antibody positive rate in MG was the highest,and it was significantly different from other neurological diseases group and normal control group(P<0.05);while the sensitivity was 55.0% and the speciality was 91.7%.RyR antibody positive rate in late-onset MG(74.4%) was significantly higher than that of early-onset MG(37.0%,(P<0.05).) There was no statistic difference in the RyR antibody positive rate between myasthenia gravis with thymoma(MGT) and myasthenia gravis with non-thymoma(nMGT)(P>0.05).The severity of MG was graded using a modified Osserman scale.It didn't correlate with the positive rate and serum obsorbance of RyR antibodies(P>0.05).Conclusions RyR antibodies occurred more frequently in late-onset MG and it had higher sensitivity and speciality for diagnosing MG.